Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns Christian S. Hansen, PhD, Martin Dufva,

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Presentation transcript:

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns Christian S. Hansen, PhD, Martin Dufva, PhD, Katrine L. Bøgh, PhD, Eric Sullivan, PhD, Jigar Patel, PhD, Thomas Eiwegger, MD, Zsolt Szépfalusi, MD, Morten Nielsen, PhD, Anders Christiansen, PhD Journal of Allergy and Clinical Immunology Volume 138, Issue 6, Pages 1728-1730 (December 2016) DOI: 10.1016/j.jaci.2016.06.019 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Effect of alanine substitution in Ara h 1 on antibody binding. The relative effect of substituting a given residue (x-axis) to an alanine is reported as 0% to 100% loss of signal compared with the signal of the native 12-mer peptide; that is, a high substitution effect signifies that the residue is important for antibody binding. Each row indicates IgE or IgG4 signal from patient samples P1 through P6 at a certain year of sampling (eg, P1_06 is patient 1 sampled in 2006). Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Alanine substitution effect for a sample epitope in Ara h 1 is depicted at high resolution. See legend of Fig 1 for additional details. Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E5 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E6 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E7 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E8 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E9 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E10 Journal of Allergy and Clinical Immunology 2016 138, 1728-1730DOI: (10.1016/j.jaci.2016.06.019) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions