Location of Pheromone Production and / or Expression in Red-sided garter snakes (Thamnophis sirtalis parietalis) By: Molly Geranio June 2016.

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Presentation transcript:

Location of Pheromone Production and / or Expression in Red-sided garter snakes (Thamnophis sirtalis parietalis) By: Molly Geranio June 2016

Abstract Pheromone communication in vertebrates -not much is known Collected red-sided garter snake, Thamnophis sirtalis parietalis -top three layers of epidermis studied throughout hibernation Statistically significant decrease in thickness of beta keratin layer

beta keratin alpha keratin stratum germinativum

Why snakes? Scientifically: red-sided garter snakes are great for studying pheromones lots of them available pheromones are distinct Personally: I think snakes are super awesome and adorable they are cute they are cuddly

Materials and methods 50 adult female red-sided garter snakes collected -Manitoba, Canada Placed in 5 groups: 0 weeks, 4 weeks, 8 weeks, 16 weeks, spring emergence Tissue prepped, stained, and imaged Statistical analysis run to compare groups

Results Beta keratin layer comparison -Statistically significant difference in thickness across all time periods throughout hibernation

Results Beta keratin layer regression analysis -Statistically significant decrease in thickness throughout hibernation

Results Alpha keratin layer comparison - Not statistically significant

Results Alpha keratin layer regression analysis - Not statistically significant

Results Stratum germinativum layer comparison - Not statistically significant

Results Stratum germinativum layer regression analysis - Not statistically significant

Discussion Alpha keratin and stratum germinativum -may be involved but irrelevant to changes in thickness Beta keratin layer -only layer with statistically significant changes in thickness -less beta keratin could mean increased permeability -pheromones may be able to reach surface more easily

acknowledgements Dr. Michael LeMaster, Thesis Adviser Dr. Gavin Keulks, Honors Program Director My parents

references http://www.reptilesmagazine.com/Snakes/Information-News/Snakes-Make- the-Perfect-Comic-Characters/ http://rebloggy.com/post/snake-noodle-ball-python-snek-snoot- champagne-ball-python/120869814088 http://rebloggy.com/post/art-cute-comic-snake-noodle-ball-python-snek- snoot/119152552928 https://www.tumblr.com/search/snake%20comic https://www.pinterest.com/lquinolima/cute-comics/