Supplementary file 1. Construct design of tagged Wnt proteins.

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Supplementary file 1. Construct design of tagged Wnt proteins. 1. Tg-Wnt3a(mouse) MLDASGCSWAMWTWALLQLLLLVGPGGCGRGYPGQYPGQYPGQYPGQYPGQVRENLYFQGKDGSSYPIWWSLAVGP.... signal(nidogen) Target tag Tev mature mouse Wnt3a(19-352) 2. Tg-Wnt5a(mouse) MLDASGCSWAMWTWALLQLLLLVGPGGCGRGYPGQYPGQYPGQYPGQYPGQVRENLYFQGKDGSNSWWSLGMNNP.... signal(nidogen) Target tag Tev mature mouse Wnt5a(38-380) 3. PA-Wnt3a(mouse) MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSSYPIWWSLAVGP.... signal(prolactin) PA tag Tev mature mouse Wnt3a(19-352) All PA-Wnt1~16(human) constructs signal(prolactin) PA tag Tev mature human Wnts 4. PA-hWnt1 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSANSSGRWWGIVNV.... mature human Wnt1 (28-370) 5. PA-hWnt2 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSSWWYMRATGGSSR.... mature human Wnt2 (26-360) 6. PA-hWnt2B2 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSARVDTSWWYIGALG.... mature human Wnt2B2 (54-391) 7. PA-hWnt3 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSGYPIWWSLALGQQYT.... mature human Wnt3 (22-355) 8. PA-hWnt3a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSSYPIWWSLAVGPQYS.... mature human Wnt3a(19-352) 9. PA-hWnt4 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSASNWLYLAKLSSVGSI.... mature human Wnt4 (22-351) 10. PA-hWnt5a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSQVVIEANSWWSLG.... mature human Wnt5a(38-380) 11. PA-hWnt5b MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSQLLTDANSWWSLA.... mature human Wnt5b(18-359) 12. PA-hWnt6 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSLWWAVGSPLVMDP.... mature human Wnt6(25-365) 13. PA-hWnt7a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSLRIGGFSSVVALGAS.... mature human Wnt7a (22-349) 14. PA-hWnt7b MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSGALSSVVALGANII.... mature human Wnt7b (24-349) 15. PA-hWnt8a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSASAWSVNNFLITGP.... mature human Wnt8a (20-351) 16. PA-hWnt8b MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSHSWSVNNFLMTGPK.... mature human Wnt8b (21-351) 17. PA-hWnt9a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSAAYFGLTGSEPLT.... mature human Wnt9a (28-365) 18. PA-hWnt9b MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSASYFGLTGREVLTPF.... mature human Wnt9b (22-357) 19. PA-hWnt10a MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSMPRSAPNDILDLRLPP.... mature human Wnt10a(36-417) 20. PA-hWnt10b MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSSRALSNEILGLKLPGEPP.... mature human Wnt10b(24-389) 21. PA-hWnt11 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSGIKWLALSKTPSALALN.... mature human Wnt11(24-354) 22. PA-hWnt16 MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSGSMGGVAMPGAEDDVVRENLYFQGKDGSGNWMWLGIASFGVPE.... mature human Wnt16(29-365) Supplementary file 1. Construct design of tagged Wnt proteins. Amino acid sequence of the N-terminal regions for the all tagged Wnt protein constructs used in this study, starting at the initiation Met.