From: Engraftment of Adult Neural Progenitor Cells Transplanted to Rat Retina Injured by Transient Ischemia Invest. Ophthalmol. Vis. Sci.. 2003;44(7):3194-3201. doi:10.1167/iovs.02-0875 Figure Legend: (A–C) GFP-labeled axons in the optic nerve. (A) Numerous GFP-labeled cells and axons (some with apparent growth cones) were present at the optic nerve head. Some processes extended into the proximal optic nerve. (B) Confocal image of proximal optic nerve immunostained with an antibody to the 200-kDa neurofilament protein (red). GFP-labeled processes (green) intermingled with host axons (red). (C) Confocal image of proximal optic nerve immunostained with an antibody to growth-associated protein (GAP-43, red). Many GFP-containing cells and processes (green) also contained GAP-43 (yellow, arrows). (D) Differential interference contrast image of proximal optic nerve at the level of the lamina cribrosa (LC). Labeled processes were immunostained with an antibody to GFP using the avidin-biotin technique. Occasional processes were observed that had penetrated the entire thickness of the lamina cribrosa (arrow). (E–G) Control studies of GFP-expressing neural progenitor cell transplantation in the rat retina. (E) Low-power fluorescence photomicrograph showing GFP-expressing neural progenitor cells in the subretinal space (✶) 2 weeks after transplantation without ischemic injury. Very limited engraftment was observed. (F) Low-power fluorescence photomicrograph showing GFP-expressing neural progenitor cells confined to the vitreous chamber 2 weeks after transplantation. Cells were delivered to the vitreous chamber immediately after 90 minutes of ischemia. Labeled cells failed to penetrate the inner limiting membrane (arrow). (G) Low-power fluorescence photomicrograph showing GFP-expressing neural progenitor cells in the subretinal space and outer retina. Cells were transplanted into the subretinal space (✶) 72 hours after 90 minutes ischemic injury. Only minimal engraftment was observed, perhaps because of the dissipation of inductive cues by this late postischemic time point. All sections were counterstained with propidium iodide. Scale bars, 50 μm. Date of download: 11/10/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.