From: Quercetin and Resveratrol Decrease the Inflammatory and Oxidative Responses in Human Ocular Surface Epithelial Cells Invest. Ophthalmol. Vis. Sci.. 2015;56(4):2709-2719. doi:10.1167/iovs.15-16595 Figure Legend: Effect of quercetin (QCT), resveratrol (RES) and QCT+RES on UV-B-induced intracellular reactive oxygen species (ROS) production. IOBA-NHC and HCE cells were pretreated with QCT (0.5, 1, 5, 10, 15, 20, and 25 μM), RES (0.5, 1, 5, 10, 25, and 50 μM), 0.5 μM QCT + 5 μM RES, or vehicle (0.5% ethanol) for 1 hour. After that, cells were loaded with 10 μM H2DCF-DA solution for 30 minutes and subsequently treated with QCT, RES, QCT+RES, or vehicle and exposed to 107.25 mJ/cm2 UV-B light (black squares). Control cells were not irradiated (white circles). After 1 hour of culture, intracellular fluorescence intensity was measured. QCT did not decrease UV-B-stimulated ROS production significantly (although ROS levels were similar to those in unexposed IOBA-NHC cells) (A), whereas 50 μM RES decreased significantly UV-B-induced ROS production by conjunctival epithelial cells (B). On the other hand, both QCT and RES decreased UV-B-induced ROS production by HCE cells at 0.5 and 25 μM, respectively (D, E). The combination of QCT+RES did not decrease ROS production significantly for either IOBA-NHC or HCE cells (C, F). Nevertheless, there were not significant differences between UV-B-exposed cells and unexposed cells (both treated with QCT+RES) for either IOBA-NHC or HCE cells. Data are presented as relative fluorescence units (RFU) normalized to micrograms of total protein (μg protein) from three independent experiments ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, compared to control cells; +P < 0.05, ++P < 0.01, +++P < 0.001, compared to vehicle-treated stimulated cells. Date of download: 11/10/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.