From: Evaluation of the Toxicity of Intravitreally Injected PLGA Microspheres and Rods in Monkeys and Rabbits: Effects of Depot Size on Inflammatory Response.

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Invest. Ophthalmol. Vis. Sci ;57(11): doi: /iovs Figure Legend:
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From: Evaluation of the Toxicity of Intravitreally Injected PLGA Microspheres and Rods in Monkeys and Rabbits: Effects of Depot Size on Inflammatory Response Invest. Ophthalmol. Vis. Sci.. 2017;58(10):4274-4285. doi:10.1167/iovs.16-21334 Figure Legend: Clinical and histopathological findings in NHPs administered PLGA microspheres. (A) Microspheres visible in the vitreous via OCT with associated retinal image cut out in a high dose (12.5 mg/eye) female at week 21. (B) Inferior retinal detachment, likely secondary to a foreign body response to the test article in a male middose (10 mg/eye) animal at week 17. (C) Fundus photograph of a middose male at week 21 showing marked clumping of microspheres over the macula. (D) Fluorescein angiogram of the same mid dose (10 mg/eye) eye taken on the same day showing a clear shadow due to the clumped microspheres. (E) Fundus photography of a mid-dose (10 mg/eye) animal showing prominent nerve head swelling and blurred disk margins. Note the hazy media and the presence of a single microsphere inferotemporally. (F) Foreign body response within the vitreous cavity in a high dose animal (12.5 mg/eye) at week 22. A mature granuloma (G), comprised of large numbers of macrophages and multinucleate giant cells, surrounding both individual beads and a central focus of necrosis (N), was adherent to the pars plana of the ciliary body (C). Variably-degraded beads were encompassed by mature fibrous connective tissue containing infiltrates of mononuclear inflammatory cells. (G) Higher magnification of the foreign body response showing multinucleate giants cells (M), fibrosis (F) and necrosis (N). (H) High magnification of the foreign body response showing encapsulated test article (TA), fibrosis (F), and multinucleate giant cells (M). Date of download: 11/11/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.