DNA and The Language of Life

Genes are Made of DNA Scientist discovered that DNA was a chemical inside the nucleus Experiments were by Biologist: Frederick Griffith Oswald Avery – worked on Griffith’s experiments Worked on bacteria to prove DNA was the reason for heredity. Oswald Avery 1944 Frederick Griffith 1928

Genes are Made of DNA Further experiments by: Alfred Hershey Martha Chase Worked with viruses to support Avery’s work

Genes are Made of DNA Virus – a package of nucleic acid wrapped in protein Not made of cells They can only reproduce by infecting a living cell Bacteriophage – a virus that infects bacteria

The Building Blocks of DNA Nucleic Acids The Building Blocks of DNA Deoxiribonucleic Acid – this where genetic information is stored DNA is a kind of nucleic acid, a polymer built from monomers called nucleotides Ribonucleic Acid (RNA) – another group of nucleotides, plays a role in protein synthesis. Nucleotides – are the building blocks (monomer) of nucleic acid polymer Only 4 types of nucleotides make – up DNA

DNA - Nucleotides Each DNA molecule has three parts:

DNA - Nucleotides Nitrogenous Base – 4 nucleotides found in DNA differ only in their nitrogenous bases Pyrimidines: single ring structure Thymine (T) Cytosine (C) Purines: double ring structure Adenine (A) Guanine (G)

Nucleotides are joined by covalent bonds DNA - Strands Nucleotides are joined by covalent bonds Connect one nucleotide to the phosphate group of the next This pattern (Sugar-Phosphate-Sugar-Phosphate) This is called Sugar – Phosphate Backbone Nitrogenous bases (AG ; CT) line – up along this backbone Nucleotides of a nucleic acid polymer can combine in many different sequences Sequences are essentially unlimited

DNA - Structure During the 1950’s scientist Rosalind Franklin produces using an X-ray (photograph) called : X-ray Crystallography – Provided clues as to the shape and dimensions of the DNA molecule

The Double Helix Double Helix – Model created by Watson and At more or less the same time scientist: James Watson Francis Crick Using Franklin’s X-ray crystalography to provide clues to their discovery Double Helix – Model created by Watson and Crick; in which two strands of nucleotides wound about each other Sugar phosphate backbones on the outside; Nitrogenous base on the inside.

The Double Helix

Complementary Base Pairs Base Pairing Rules A with T C with G AT CG (Coral Gables)

DNA Replication When a cell divides; a complete set of genetic instructions are created for each cell Genetic material uses the template principle to make more DNA Watson and Crick’s model for DNA allowed for the hypothesis of specific base pairing A – T C - G If you know one strand of DNA you can determine the sequence on the other

Called Daughter Strands DNA Replication During DNA copying: Two strands of the double helix separate Each strand acts like a picture negative Nucleotides line up one at a time across the existing strand (as per base pairing rule) Enzymes link the nucleotides together to form two new DNA strands Called Daughter Strands

DNA Replication

DNA Replication More than a dozen enzymes are involved in DNA replication DNA Polymerases – Name of enzymes involved in DNA replication Make covalent bonds between nucleotides of the new DNA strand This happens really fast and it is really accurate An error occurs in only one of a billion nucleotides DNA Polymerases

DNA Replication Origins of Replication - Specific sites where replication begins The copying occurs outward in both directions creating replication bubbles. Daughter Strands grow on the inside

DNA Replication In Eukaryotic Cells this can occur at the same time in different location They eventually join. This shortens replication time.

DNA Replication DNA replication occurs before cell division This ensures that cells carry the same genetic information This is also the method for inheritance.

Information for Making Protein The molecular basis for phenotype (physical characteristics) lies in proteins “One Gene-One Enzyme” Hypothesis One gene dictates the production of a specific enzyme Hypothesis developed by geneticists George Beadle and Edward Tatum in the 1940’s Since then the hypothesis has evolved to: “one gene – one polypeptide”

DNA to RNA = Protein As we know a gene is a sequence of bases along DNA chain A gene is like a sentence Specific strings of bases make-up a gene sentence

RNA (ribonucleic acids) Any nucleic acid whose sugar is ribose rather than deoxyribose (DNA) Another difference is that the Nitrogenous Base Thymine is replaced by Uracil (U) Similar to thymine Also Pair with Adenine RNA forms single strands NOT a double helix (like DNA) RNA can twist

RNA Transcription – The process of converting a DNA sequence to a form a single stranded RNA molecule. RNA molecule leaves the nucleus and is involved with the making of protein

RNA Genetic Translation – Converts nucleic acid language into amino acid language Codon – A 3 – Base “word” that codes for one amino acid This is how the flow of information from gene to protein is based on Several codons form a sentence – that translates into polypetide

Triplet Code There are specific codons to represent amino acid Some amino acids are coded by more than one codon But no codon represents more than one amino acid

This coding is shared by ALL organisms (almost all) Triplet Code There is also a code for stop These come at the end of a sequence There are sequence that do not code form an amino acids This coding is shared by ALL organisms (almost all) Evidence of Evolution??????

From Gene to Protein Transcription: DNA RNA Three Types of RNA messenger RNA (mRNA) Transcribed from a DNA template Only one DNA strand serves as a template for forming mRNA

From Gene to Protein - Steps 1st – DNA strand separates – This is where transcription will start 2nd – RNA bases pair with complementary DNA bases (same as DNA replication, except Uracil)

From Gene to Protein RNA Polymerase – An enzyme; links RNA nucleotides together. Still in second step; specific sequences of DNA nucleotides tell polymerase where to begin and end the transcribing process RNA Polymerase

From Gene to Protein Introns – non-coding regions in mRNA Only found in eukaryotic cells (most plants and animals) Exons – parts of mRNA translated or expressed. Before RNA leaves the nucleus the introns are removed and exons are joined together RNA Splicing – the process of removing introns and joining exons

From Gene to Protein Translation: RNA to Protein Requires enzymes and ATP Transfere RNA (tRNA) – translates the 3 – Letter codons of mRNA to the amino acids that make up proteins.

From Gene to Protein - Translation Anticodon Located at one end of the folded tRNA molecule It is a specific triplet of bases Are complementary (or matched up with) a specific codon in m RNA Recognition follows base pair rules (AU / CG) At the other end is the site where a particular amino acid recognizes both tRNA and its amino acid partner and links both together. Requires

From Gene to Protein - Translation Ribosome – coordinates the functioning of mRNA and tRNA Made of Two Subunits: Both made of proteins and rRNA (ribosomal) rRNA – acts like a “vise”; holds mRNA and tRNA molecules close together. P - Site and A – Site P – Site - a location on the t RNA molecule that allows for the polypeptide strand to hold together. A – Site – a location on the tRNA molecule that allows for amino acids to hold together. Ribosomes connect Amino Acid to polypeptide chain

Do not code for amino acid The Process 1st Step in Translation All parts needed are brought together mRNA First tRNA with amino acid attached Two subunits of a ribosome The start codon AUG Next Step Amino Acids are added one by one to the chain of amino acids. Each addition occurs in a 3-step process Continues till you reach a stop codon UAA UAG UGS Do not code for amino acid

The Process - continued Finally The complete polypeptide (several 100 amino acids) is set free by hydrolysis from tRNA A single ribosome can make an average polypeptide in less than a minute. This is happening all over the cell at the same time.

Protein Synthesis

Mutations

Mutations Any change in the nucleotide sequence of DNA They can involve large regions of a chromosome or just a single nucleotide pair

Two Catagories of Mutations Base Substitution – the replacement of one base or nucleotide with another Usually causes no change to the protein But sometimes change results in a change that affects protein function Sometime drastically Because several amino acids have more than one codon it makes it possible that a mutation have no effect on a protein

Mutations - Base Substitution Silent Mutation – a mutation to DNA causes mRNA codon to change from GAA to GAG (no change in protein Some changes for amino acids have similar chemical properties – THUS: No Change

Mutations Base Insertions or Deletion Has a more significant effect Because mRNA is read as a series of triplet grouping, adding or subtracting Will alter grouping of genetic message

Mutations - Base Insertions or Deletions Causes: Mutations may occur when errors are made during DNA replication or when errors are made during chromosome cross-over Mutagens – a physical or chemical agent that cause mutations High energy radiation (x-rays and uv rays) One type of chemical mutagen are chemicals that are similar to normal DNA bases but causes incorrect base-pairing

Mutations Can be harmful Can be helpful in certain environments If mutation is present in gametes it can be passed on to its offspring Mutations are the ultimate source of genetic diversity.