Jack Lawler, Wei-Min Miao, Mark Duquette, Noël Bouck, Roderick T

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Thrombospondin-1 Gene Expression Affects Survival and Tumor Spectrum of p53- Deficient Mice Jack Lawler, Wei-Min Miao, Mark Duquette, Noël Bouck, Roderick T. Bronson, Richard O. Hynes The American Journal of Pathology Volume 159, Issue 5, Pages 1949-1956 (November 2001) DOI: 10.1016/S0002-9440(10)63042-8 Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 1 Survival of mice with various genotypes. The genotypes are identified as TTpp (Thbs1+/+,p53−/−), ttpp (Thbs1−/−,p53−/−), TTPp (Thbs1+/+,p53+/−), and ttPp (Thbs1−/−,p53+/−). The number of mice with each genotype that were followed for survival and the mean survival is given in Table 1. The American Journal of Pathology 2001 159, 1949-1956DOI: (10.1016/S0002-9440(10)63042-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 2 Analysis of LOH. DNA from tumors from nine mice that were heterozygous for the p53-null allele was amplified with primers for the p53 wild-type (+) and null (−) alleles (lanes 1 to9). The PCR products were Southern blotted with the wild-type PCR product. Lanes 1,3, 4, 6, 7, and8 were identified as having lost heterozygosity based on the absence or underrepresentation (lane 4) of the p53 wild-type allele.Lane 10 shows the expression of the p53 wild-type and null alleles in tail DNA from a p53 heterozygous mouse. The American Journal of Pathology 2001 159, 1949-1956DOI: (10.1016/S0002-9440(10)63042-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 3 Growth of B16F10 tumors in TSP1-deficient and wild-type mice. Tumor cells (2.5 × 105) were inoculated subcutaneously on the backs of C57BL/6J wild-type (filled circles) or Thbs1-null mice (open squares). Tumor volume was determined with a caliper and calculated by the formula 0.52 × width2 × length. The American Journal of Pathology 2001 159, 1949-1956DOI: (10.1016/S0002-9440(10)63042-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions

Figure 4 Histological analysis of B16F10 tumors in wild-type (filled bar) and TSP1-null (hatched bar) mice. Vessel counts (A) were determined on CD31-stained sections, apoptotic index (B) was determined on terminal dUTP nick-end labeling-stained sections and proliferative index (C) was determined on proliferating cell nuclear antigen-stained sections. Theasterisk indicates statistically significant differences (P < 0.005). The American Journal of Pathology 2001 159, 1949-1956DOI: (10.1016/S0002-9440(10)63042-8) Copyright © 2001 American Society for Investigative Pathology Terms and Conditions