Figure 1. Colony PCR of randomly selected clones of the whole genome M

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Figure 1. Colony PCR of randomly selected clones of the whole genome M Figure 1. Colony PCR of randomly selected clones of the whole genome M. tuberculosis phage library. Lane M is 100 bp ladder and 1–33 are PCR amplicons of library clones. From: Identification of unique essential proteins from a Mycobacterium tuberculosis F15/LAM4/KZN phage secretome library Pathog Dis. 2017;75(1). doi:10.1093/femspd/ftx001 Pathog Dis | © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Figure 2. (A) Selective disassembly of phage particles display no or non-secretory protein. PP: phage particles prepared from M. tuberculosis phage library; SS: DNA released from sarcosyl-sensitive phage particles; DT: DNase I treatment results showing almost complete removal or digestion of phage DNA released during SS step. (B) PCR amplicons of randomly selected clones of the phage secretome sub-library. From: Identification of unique essential proteins from a Mycobacterium tuberculosis F15/LAM4/KZN phage secretome library Pathog Dis. 2017;75(1). doi:10.1093/femspd/ftx001 Pathog Dis | © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Figure 3. Distribution of the functional categories of 86 identified ORFs from the F15/LAM4/KZN phage displayed secretome. The number of proteins in the different functional categories were: cell wall and cell processes (34), conserved hypothetical proteins (16), intermediary metabolism and respiration (17), lipid metabolism (8), information pathways (4), PE/PPE family proteins (3), virulence, detoxification and adaptation (2), regulatory proteins (1) and unknown (1) function according to UniProt. Functional group codes were obtained from the TubercuList database web server except for the unknown protein that was confirmed by the UniProt server. From: Identification of unique essential proteins from a Mycobacterium tuberculosis F15/LAM4/KZN phage secretome library Pathog Dis. 2017;75(1). doi:10.1093/femspd/ftx001 Pathog Dis | © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com