Heat-transfer method-based cell culture quality assay through cell detection by surface imprinted polymers Kasper Eersels, Bart van Grinsven, Mehran Khorshid,

Slides:

Advertisements

Similar presentations

(and briefly, Electrodeposition)

Advertisements

WP4 Organic crystal deposition Mojca Jazbinsek Rainbow Photonics AG SOFI meeting Karlsruhe

Adhesive bonding Ville Liimatainen Contents Introduction – Adhesive bonding – Process overview – Main features Polymer adhesives Adhesive.

Carbon nanotube field effect transistors (CNT-FETs) have displayed exceptional electrical properties superior to the traditional MOSFET. Most of these.

Chapter 7b Fabrication of Solar Cell. Different kind of methods for growth of silicon crystal.

Application of Silicon Nanowire in Biosensor Student: HongPhan ID: Professor: Cheng-Hsien Liu.

Laser etching of GaN Jonathan Winterstein Dr. Tim Sands, Advisor.

ELECTRICAL POROUS SILICON MICROARRAY FOR DNA HYBRIDIZATION DETECTION M. Archer*, D. Persaud**, K. D Hirschman**, M. Christophersen* and P. M Fauchet* *Center.

Nanoimprint II. NIL Technology sells stamps for nanoimprint lithography (NIL) and provides imprint services. Stamps made in Siliocn, Quartz, and Nickel.

Atmospheric Pressure Atomic layer deposition (AP – ALD)

Workshop for NFF Nanoimprint System NFF MA6 Nanoimprint Upgrade.

Isolation of Rare Circulating Tumor Cells Sunitha Nagrath et al. Presentation.

Cell Size Surface area : Volume Ch 8.2 pg 201. Cell Size Limits Are whale cells the same size as sea stars cells? Yes!

NANOSCALE LITHOGRAPHY MICHAEL JOHNSTON 4/13/2015.

McGill Nanotools Microfabrication Processes

Microcantilevers III Cantilever based sensors: 1 The cantilever based sensors can be classified into three groups (i)General detection of any short range.

New approach for the determination of the hybridization efficiency of ssDNA nanopatches 1. CNR-INFM, Laboratorio Nazionale TASC, Trieste, Italy 2. SISSA,

SEMINAR ON IC FABRICATION MD.ASLAM ADM NO:05-125,ETC/2008.

Robert E. Thorne Cornell University Alexander Malkin LLNL

Nanotechnology The biggest science and engineering initiative since the Apollo program.

Techniques for Synthesis of Nano-materials

Chapter 7 Lesson 3. Physical Change A change in size, shape, form, or state of matter Matter’s identity stays the same Matter does not become something.

Temperature Sensitive Micro-electro-mechanical Systems Amy Kumpel Richard Lathrop John Slanina Haruna Tada Tufts University TAMPL REU 1999.

Disturbing (polymers and) proteins with Atomic Force Microscopy José L. Toca-Herrera ETSEQ, 02/06/2004.

Accelerated Life Test of High Brightness Light Emitting Diodes 陳詠升.

Microcontact Printing

Enhanced metrology, cleaning and repair technologies for micro and nano-scale defects on large area substrates The NanoMend project has received funding.

LITHOGRAPHY IN THE TOP-DOWN PROCESS - NEW CONCEPTS

Lithography. MAIN TYPES OF LITHOGRAPHY: * Photolithography * Electron beam lithography –X-ray lithography –Focused ion beam lithography –Neutral atomic.

E-beam Size-Dependent Self- Assembly Protein Array.

Fabrication of Suspended Nanowire Structures Jason Mast & Xuan Gao

Spencer/Ghausi, Introduction to Electronic Circuit Design, 1e, ©2003, Pearson Education, Inc. Chapter 3, slide 1 Introduction to Electronic Circuit Design.

Roll-to-Roll Fabrication Technology for Organic Semiconductors Jae Young Choi Nov. 13, 2007.

Lithography in the Top Down Method New Concepts Lithography In the Top-Down Process New Concepts Learning Objectives –To identify issues in current photolithography.

Department of Chemistry , SungKyunKwan University

Updates of Iowa State University S. Dumpala, S. Broderick and K. Rajan Sep – 18, 2013.

Yongshik Park EE235 04/14/08. Nanoimprint Lithography (1994) Imprint mold with 10nm diameter pillars 10nm diameter holes imprinted in PMMA 10nm diameter.

Liquid Flame Spray Deposition on Temperature Sensitive Substrates Antti Toropainen

*) Hasselt University, Diepenbeek, Belgium Detection of the peanut allergen Ara h 1 by electrochemical impedance spectroscopy and the heat-transfer method.

Spatial ALD Ismo Heikkinen

Mukhtar Hussain Department of Physics & Astronomy King Saud University, Riyadh

Developing a Versatile Platform for Nanoscale Materials Characterization Julia Bobak, Daniel Collins, Fatemeh Soltani, David W. Steuerman Department of.

Microcontact Printing (Stamping) Andrew van Bommel February 7 th, 2006.

First steps towards the PhD thesis

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

Photolithography PEOPLE Program.

Stacking of Quasi 2D Transition Metal Dichalcogenides

CMOS Fabrication CMOS transistors are fabricated on silicon wafer

Kathryn Rose Verfaillie with Dr. Nivedita Gupta and Brian Zukas

Lecture 6 Fundamentals of Multiscale Fabrication

Temperature Sensors on Flexible Substrates

BY SURAJ MENON S7,EEE,61.

Preparation of Surface for Biomolecule Immobilization

Development and characterisation of laser-patterned polymer substrates for controlling cell growth Michael Irving.

Multilevel resistive switching memory based on GO/MoS2/GO stack

Molding PDMS Channels and an Embedded Detector Chamber

Soft Lithography Xia, Y.; Whitesides, G. M.

Cell Size Limitation Notes

LITHOGRAPHY Lithography is the process of imprinting a geometric pattern from a mask onto a thin layer of material called a resist which is a radiation.

Nanocharacterization (III)

Printable Metal-Polymer Conductors for Highly Stretchable Bio-Devices

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Thermal behavior of the LHCb PS VFE Board

Cell Size Surface area : Volume

Molecularly imprinted polymers

Products > ARPE-19 Transfection Reagent (Retinal Epithelium)

Cell Size and Growth Chapter 10-1 Pg. 241.

Fig. 1 Schematic view and characterizations of FGT/Pt bilayer.

Presentation transcript:

Heat-transfer method-based cell culture quality assay through cell detection by surface imprinted polymers Kasper Eersels, Bart van Grinsven, Mehran Khorshid, Veerle Somers, Thomas Cleij, Ronald Thoelen, Patrick Wagner

Sediment cells on a home-made silicone stamp (PDMS) Surface Imprinting? Sediment cells on a home-made silicone stamp (PDMS) Spin coating removes excess buffer solution and target cells form a monolayer on the stamp Hayden, O. and Dickert, F.L. Adv. Mater. 2001, 13, 1480-1483.

Spin coating of a semi-cured PU layer onto aluminum substrate Surface Imprinting? Removal of the stamp and cells creates a SIP layer imprinted for target cell type Spin coating of a semi-cured PU layer onto aluminum substrate Stamping of the cells in the PU layer, curing the layer overnight at 65°C PU: polyurethane

SIP Surface Characterization Optical microscopy Atomic Force Microscopy K. Eersels, B. van Grinsven et al. ACS AMI 2013, 5(15), 7258-7267.

Read-Out: the Heat-Transfer Method SIP Rth = 𝑻𝟏 −𝑻𝟐 𝑷 B. van Grinsven et al. ACS Nano 2012, 6(3), 2712-2721.

Read-Out: the Heat-Transfer Method Microcavities form preferential heat channels Cell binding causes an increase in thermal resistance (Nakano et al.) Heat is forced to transfer over thicker, non-imprinted region Measured as a decrease in T2  Increase in Rth K. Eersels, B. van Grinsven et al. ACS AMI 2013, 5(15), 7258-7267.

Detection of Circulating Tumor Cells? Selective discrimination between tumor cells and healthy cells Sensitivity limits applicability in CTC detection  Improve sensitivity and find other applications K. Eersels, B. van Grinsven et al. ACS AMI 2013, 5(15), 7258-7267.

Experiment RWTH Aachen: problem with ZR-75-1 cells, grow pattern changed STR DNA profile: over-passaging led to cross- contamination with a faster growing cell line Reproduce circumstances: grow both cell lines for 3 months and about 25 passages in the same lab Analyze ZR-75-1 cells with SIP-based assay on HTM

Results At passage number 15 “something” happens Effect increases with passage number At passage 22 Rth minimum reached  STR DNA profile: cross- contamination!

Conclusions State-of-the-art devices (FACS) or assays (STR DNA profile) too slow and expensive for routine screening HTM can offer a low-cost and fast alternative Sensitivity is sufficient Limited to changes at the membrane Setup needs to be tested for other cell lines

The end? Questions?