Agreement Between Home-Based Measurement of Stool Calprotectin and ELISA Results for Monitoring Inflammatory Bowel Disease Activity Anke Heida, Mariska.

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Agreement Between Home-Based Measurement of Stool Calprotectin and ELISA Results for Monitoring Inflammatory Bowel Disease Activity Anke Heida, Mariska Knol, Anneke Muller Kobold, Josette Bootsman, Gerard Dijkstra, Patrick F. van Rheenen Clinical Gastroenterology and Hepatology Volume 15, Issue 11, Pages 1742-1749.e2 (November 2017) DOI: 10.1016/j.cgh.2017.06.007 Copyright © 2017 AGA Institute Terms and Conditions

Figure 1 IBDoc sampling and measurement. Adapted with permission from Bühlmann Laboratories AG.23 Clinical Gastroenterology and Hepatology 2017 15, 1742-1749.e2DOI: (10.1016/j.cgh.2017.06.007) Copyright © 2017 AGA Institute Terms and Conditions

Figure 2 Study flow indicating 3 calprotectin measurements from the same bowel movement. ELISA, enzyme-linked immunosorbent assay. Clinical Gastroenterology and Hepatology 2017 15, 1742-1749.e2DOI: (10.1016/j.cgh.2017.06.007) Copyright © 2017 AGA Institute Terms and Conditions

Figure 3 Bland-Altman plot showing difference against mean. (A) IBDoc and (enzyme-linked immunosorbent assay (ELISA) measurements. (B) IBDoc and Quantum Blue measurements. The pink zone corresponds with our predefined limits of agreement, which were arbitrary set at ±100 μg/g for the lower calprotectin range, and ±200 μg/g for the higher range. The outer lines correspond with the 95% limits of agreement (SD, 1.96). Clinical Gastroenterology and Hepatology 2017 15, 1742-1749.e2DOI: (10.1016/j.cgh.2017.06.007) Copyright © 2017 AGA Institute Terms and Conditions

Figure 4 Scatterplot showing calprotectin readings with IBDoc against enzyme-linked immunosorbent assay (ELISA) method. Concordance is defined as calprotectin levels in the same category (<250, 250–500, >500 μg/g) for both IBDoc and ELISA. Clinical Gastroenterology and Hepatology 2017 15, 1742-1749.e2DOI: (10.1016/j.cgh.2017.06.007) Copyright © 2017 AGA Institute Terms and Conditions

Supplementary Figure 1 Overview of transport time. Clinical Gastroenterology and Hepatology 2017 15, 1742-1749.e2DOI: (10.1016/j.cgh.2017.06.007) Copyright © 2017 AGA Institute Terms and Conditions