Figure Legend: From: Role of ErbB2 in Corneal Epithelial Wound Healing

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Figure Legend: From: Role of ErbB2 in Corneal Epithelial Wound Healing Invest. Ophthalmol. Vis. Sci.. 2004;45(12):4277-4283. doi:10.1167/iovs.04-0119 Figure Legend: Time course of tyrosine phosphorylation of erbB2 in wounded THCE cells. THCE cells were cultured in 100-mm dishes and starved overnight. Cells were extensively injured by sequence comb scratching and incubated for different time points after wounding (p.w.). Wounded THCE cells were lysed, and 600 μg cell lysate from each time point was immunoprecipitated (IP) with 10 μg agarose-conjugated pY20 (phosphotyrosine-specific) antibodies, subjected to SDS-PAGE, and immunoblotted with anti-erbB2 antibody (top). To determine the cellular level of erbB2, 20 μg cell lysate from each sample was subjected to Western blot analysis with anti-erbB2 antibody (bottom). Results are representative of three independent experiments. Date of download: 11/12/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.