PCR Polymerase Chain Reaction

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Presentation transcript:

PCR Polymerase Chain Reaction a tool to generate a large amount of a gene of interest

 Polymerase Chain Reaction DNA-sequence in a plasmid Restriction of plasmid releases the DNA sequence of interest Polymerase Chain Reaction synthesizes only the gene of interest by using Forward and Reverse primers (specific small pieces of DNA) with the help of the enzyme Polymerase. All cycles follow a strict order of timed temperatures. First cDNA strand Forward primer Reverse primer Second cDNA strand Gene of Interest