From: Glucose Infusion Suppresses Surgery-induced Muscle Protein Breakdown by Inhibiting Ubiquitin-proteasome Pathway in Rats Anesthes. 2009;110(1):81-88.

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【 Supplement methods 】 Real-Time Reverse Transcription-PCR Total RNA was isolated from HRGEc using the RNeasy® Mini Kit. cDNA was synthesized using Super-Script®

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From: Glucose Infusion Suppresses Surgery-induced Muscle Protein Breakdown by Inhibiting Ubiquitin-proteasome Pathway in Rats Anesthes. 2009;110(1):81-88. doi:10.1097/ALN.0b013e318190b6c1 Figure Legend: Fig. 4. Glucose infusion suppressed the mRNA expression of atrogin-1/MAFbx (atrogin-1) and muscle ring finger-1 (MuRF1) in gastrocnemius muscles. A-F Fasted rats were subjected to laparotomy for 4 h under anesthesia. During and for 4 h after laparotomy, the rats were continuously infused with acetated Ringer’s solution without glucose (control), or containing 1% or 5% glucose. Total RNA was extracted from gastrocnemius muscles isolated 8 h after the induction of anesthesia. Total RNA (1 μg) was subjected to real-time reverse transcription-polymerase chain reaction analyses for muscle proteases and related genes. The fluorescence ratio of the cDNA of the target genes to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was calculated. Values are means ± SD, n = 10 or 7 (nonsurgical treatment group). Means with different superscripts differ significantly ( P < 0.05). Date of download: 11/12/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.