Cell and matrix morphology in articular cartilage from adult human knee and ankle joints suggests depth-associated adaptations to biomechanical and anatomical.

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Presentation transcript:

Cell and matrix morphology in articular cartilage from adult human knee and ankle joints suggests depth-associated adaptations to biomechanical and anatomical roles T.M. Quinn, H.-J. Häuselmann, N. Shintani, E.B. Hunziker Osteoarthritis and Cartilage Volume 21, Issue 12, Pages 1904-1912 (December 2013) DOI: 10.1016/j.joca.2013.09.011 Copyright © 2013 Terms and Conditions

Fig. 1 Light micrographs of normal adult human articular cartilage originating from the superficial/transitional zones (A: talar dome, B: medial tibial plateau), the upper radial zone (C: talar dome, D: medial tibial plateau), and the lower radial zone (E: talar dome, F: medial tibial plateau). In the superficial zone, chondrocytes are relatively flattened with elliptical profiles. In the transitional zone, chondrocytes are more rounded, and in the radial zone they tend to be grouped in vertical stacks (chondrons). Semi-thin (1 μm thick) sections of Epon-embedded tissue stained with Toluidine Blue O. Bar = 100 μm. Osteoarthritis and Cartilage 2013 21, 1904-1912DOI: (10.1016/j.joca.2013.09.011) Copyright © 2013 Terms and Conditions

Fig. 2 Transmission electron micrographs of normal adult human articular cartilage originating from the superficial zone (A: talar dome, B: medial tibial plateau), the transitional zone (C: talar dome, D: medial tibial plateau), the upper radial zone (E: talar dome, F: medial tibial plateau) and the lower radial zone (G: talar dome, H: medial tibial plateau). Thin (60 nm thick) sections of Epon-embedded tissue stained with uranyl acetate and lead citrate. Bar = 10 μm. Osteoarthritis and Cartilage 2013 21, 1904-1912DOI: (10.1016/j.joca.2013.09.011) Copyright © 2013 Terms and Conditions

Fig. 3 Cartilage thickness at four different anatomical sites in the human knee (MFC and MTN) and ankle (TAL and DTB; mean ± 95% confidence limits, n = 10). Star (★) indicates significantly different thicknesses between knee vs ankle cartilage (P < 0.05). Osteoarthritis and Cartilage 2013 21, 1904-1912DOI: (10.1016/j.joca.2013.09.011) Copyright © 2013 Terms and Conditions

Fig. 4 Cell volume per unit tissue volume (VV) within cartilage from four different anatomical sites in the human knee (MFC and MTN; mean ± 95% confidence limits, n = 8) and ankle (TAL and DTB; n = 10). Data were acquired from confocal microscopy of a) superficial zone, b) transitional zone, and c) radial zone cartilage. Star (★) indicates significant differences in VV between knee vs ankle cartilage, or between cartilage from convex (MFC and TAL) vs concave (MTN and DTB) articular surfaces (P < 0.05). Osteoarthritis and Cartilage 2013 21, 1904-1912DOI: (10.1016/j.joca.2013.09.011) Copyright © 2013 Terms and Conditions

Fig. 5 Pericellular (white bars) and interterritorial (black bars) matrix volume per cell within cartilage from superficial, transitional and radial tissue zones. Data were acquired from a combination of confocal and EM of a) medial tibia not meniscus-covered (MTN; n = 5) and b) talus (TAL; n = 7) cartilage. Osteoarthritis and Cartilage 2013 21, 1904-1912DOI: (10.1016/j.joca.2013.09.011) Copyright © 2013 Terms and Conditions