Root cDNA library preparation for untreated (water) and salt challenged plants (treated at 150mM NaCl for 2.0 hrs) Cloning of the library into yeast expression.

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Root cDNA library preparation for untreated (water) and salt challenged plants (treated at 150mM NaCl for 2.0 hrs) Cloning of the library into yeast expression vector and amplification of the libraries on E.coli. Library titer estimation for the constructed libraries Mobilization of the library into yeast, transformation of shuttle vector into E.coli and amplification of the library. Treatment and screening yeast transformants for response to salt and drought resistance High throughput quadrapulate screens using 96 well grids and replica printing system Isolation of yeast transformants tolerant to salt stress (or other related abiotic stress) Re-screening for salt tolerance and stress challenge tests and quality control of screening parameters Plasmid rescue and sequencing, followed by computational sequence analysis and annotation Transformation of yeast and selection of the transformants Library generation into yeast expression system