Increased systemic and adipose tissue inflammation differentiates obese women with T2DM from obese women with normal glucose tolerance  Lianne van Beek, Mirjam A. Lips, Annemieke Visser, Hanno Pijl, Andreea Ioan-Facsinay, René Toes, Frits J. Berends, Ko Willems van Dijk, Frits Koning, Vanessa van Harmelen  Metabolism - Clinical and Experimental  Volume 63, Issue 4, Pages 492-501 (April 2014) DOI: 10.1016/j.metabol.2013.12.002 Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 1 Quantification of macrophages and fat cell size determination in visceral and subcutaneous adipose tissue of obese women with NGT or T2DM. A) CD68 staining in adipose tissue showing solitary macrophages (MΦ) and crown like structures (CLS). B) Number of CLS and C) solitary or single macrophages per area of adipose tissue section as determined by immunostaining of CD68, D) Fat cell size, expressed as mean adipocyte diameter. Metabolism - Clinical and Experimental 2014 63, 492-501DOI: (10.1016/j.metabol.2013.12.002) Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 2 A–B) Expression of CD25 in CD8 and CD4 T cells (as % of CD8 or CD4 cells) and C) Expression of CD38 in CD19 B cells (as % of CD19 cells) in lean and obese women with NGT or T2DM. Metabolism - Clinical and Experimental 2014 63, 492-501DOI: (10.1016/j.metabol.2013.12.002) Copyright © 2014 Elsevier Inc. Terms and Conditions

Fig. 3 Proliferation capacity and intracellular cytokine staining of PBMCs from obese women with low or high peripheral CD8+CD25+. A) Proliferation of T cells after either PHA, IL-2, IL-7 or IL-15 stimulation. B) Percentage CD4+ or CD8+ cells that are positive for TNF-α, IFNγ, IL-6, IL-10 or IL-17A upon in vitro stimulation with PMA/ionomycin. Metabolism - Clinical and Experimental 2014 63, 492-501DOI: (10.1016/j.metabol.2013.12.002) Copyright © 2014 Elsevier Inc. Terms and Conditions