Apolipoprotein E gene polymorphisms (rs429358 and rs7412) in the healthy Bosnian population as a complement the European data. Grażyna Adler 1, Amina Valjevac A2, Emina Kiseljaković-Čengić 3, Nermin Nusret Salkić4, Karolina Skonieczna-Żydecka1, Anna Urbańska5, Miłosz Parczewski5 1Department of Gerontobiology, Pomeranian Medical University, Żołnierska 48, 71-210 Szczecin, Poland, gra2@op.pl 2Laboratory for Molecular Medicine Center for Genetics, Medical Faculty, University of Sarajevo, Cekalusa 90, 71 000 Sarajevo, Bosnia and Herzegovina 3Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo, Cekalusa 90, 71 000 Sarajevo, Bosnia and Herzegovina 4Department of Gastroenterology and Hepatology, University Clinical Center Tuzla, Trnovac bb, 75 000 Tuzla, Bosnia and Herzegovina 5Department of Infectious, Tropical Diseases, Pomeranian Medical University, Arkońska 4, 71-455 Szczecin, Poland Objectives: Background: The alleles of APOE gene such as: Ɛ2, Ɛ3, Ɛ4 have been associated with a number of diseases in different populations. The frequency of the APOE alleles varies worldwide and differences are observed between geographic locations and ethnic populations. In Europe, in Northern countries the average frequency of alleles Ɛ2, Ɛ3 and Ɛ4 is 8.5%, 75.5% and 16.0%, whereas in Middle and Western countries is 8.6%, 76.2% and 15.3%, respectively. In Southern countries the average frequency of alleles Ɛ2, Ɛ3 and Ɛ4 is 7.5%, 80,2% and 12.2%, respectively. Due to important role in metabolism and clinical importance allelic variation in different populations is analyzed to evaluate the usage of APOE in a clinical and evolutionary context. All in all, data on frequency of alleles are numerous but reports in population of Bosnia and Herzegovina are nonexistent. Therefore, the aim of this study was to estimate the allele frequency for the APOE polymorphism in healthy Bosnian subjects and to compare to data in other European countries. Methods: Methods: We recruited 170 subjects (108 female and 62 male) from Bosnia and Herzegovina, mean age of cohort was 53.0 (±5.0) years. Genotypes were determined by real-time PCR using StepOneTM Real-Time PCR System, Applied Biosystems (AB) and TaqMan SNP Genotyping Assays for two APOE polymorphisms including rs429358 and rs7412 (Life technologies, Assay ID: C_3084793_20, C_904973_10, respectively) (AB). The data were analyzed with Taq Man Genotyper Software v. 1.0.1. Electronic databases including Medline and Embase were searched from 1997 to April 2015. Results: Results: In our group the prevalence of heterozygotes: Ɛ2Ɛ3, Ɛ2Ɛ4 and Ɛ3Ɛ4 was 20.6%, 3.5% and 12.9%, respectively, while prevalence of homozygotes: Ɛ2Ɛ2, Ɛ3Ɛ3, Ɛ4Ɛ4 was 0.6%, 61.2% and 1.2%, respectively, with mean Ɛ2, Ɛ3, Ɛ4 allele frequency of 12.6%, 78.0%, 9.4%, respectively. Conclusions: Conclusions: The findings of the investigation in Bosnia show higher frequency of Ɛ2 allele than Northern, Middle and Western and Southern European countries, frequency of Ɛ3 allele was lower only in comparison with Southern countries, while frequency of Ɛ4 allele was lower than in all these regions of Europe. For frequency of Ɛ4 allele decreasing linear gradient from North to South was observed. 23rd Meeting of the Balkan Clinical Laboratory Federation Oktober 7- 9, 2015, Sarajevo, Bosnia and Herzegovina