From: In Vivo Imaging of Microscopic Structures in the Rat Retina

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Invest. Ophthalmol. Vis. Sci ;48(10): doi: /iovs Figure Legend:

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From: In Vivo Imaging of Microscopic Structures in the Rat Retina Invest. Ophthalmol. Vis. Sci.. 2009;50(12):5872-5879. doi:10.1167/iovs.09-3675 Figure Legend: Direct comparison of in vivo and ex vivo rat retinal images. (a) In vivo image in a rat retina taken with adaptive optics correction over a 0.25 NA. (b) Ex vivo histologic image acquired using a confocal microscope with a 0.3 NA objective. (c) Ex vivo histologic image acquired using a confocal microscope with a 1.2 NA objective in which details of the cell soma, dendrite, and axon structures can be seen. The in vivo image is a maximum intensity projection image generated from three separate in vivo images taken at different depths, each of which is a registered sum of 1000 frames. Both ex vivo images are maximum intensity projection images generated from a focus stack of 63 images of the ganglion cell layer. (a, c, gray rectangles) Locations in which we characterized the in vivo resolution. All images have been rescaled and contrast enhanced for display purposes. Scale bar, 20 μm. Date of download: 11/13/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.