PROJECT: Model DNA http://www.youtube.com/watch?v=CpPukndUfHk

Can you see the DNA? Can you touch it?

HOOK http://www.youtube.com/watch?v=1Q67bMYOm7E

IBE Is the DNA the same in all organisms?

J-TPS (Justify –Think Pair Share)

DNA structure is the same in all organisms

DNA is composed of nucleotides DNA is made up of a polymer (long chain) of nucleotides. Each nucleotide has three parts. A phosphate group A deoxyribose sugar A nitrogen-containing base

The Nucleotide Bases The nitrogen containing bases are the only parts in the nucleotides that are different.

White board DNA structure is the same in all organisms A. True B False

Watson and Crick determined the three-dimensional structure of DNA by building models These scientists discovered that the DNA molecule is a double helix that is made up of a sugar-phosphate backbone on the outside with bases on the inside.

Watson and Crick’s discovery was built on the work of Rosalind Franklin and Erwin Chargaff. Franklin’s x-ray images suggested that DNA was a double helix of even width. Chargaff’s rules stated that A=T and C=G.

Nucleotides always pair in the same way The base-pairing rules show how nucleotides always pair up in DNA. A pairs with T C pairs with G C G T A

The backbone is connected by covalent bonds. The bases are connected by hydrogen bonds. hydrogen bond covalent bond

G A T T A C A Practice DNA Base Pairs “Gattaca” is a science fiction movie starring Uma Thurman, Ethan Hawke, and Jude Law

G A T T A C A C T A A T G T Practice DNA Base Pairs “Gattaca” is a science fiction movie starring Uma Thurman, Ethan Hawke, and Jude Law

DNA vs. RNA DNA RNA Sugar = deoxyribose Sugar = ribose Double-stranded molecule Single-stranded molecule Thymine bonds with adenine Uracil instead of thymine

White board The following sequence belongs to AAUCGCGUAACCGGCC A. DNA B. RNA C. DNA & RNA D Not enough information is provided

White board What’s wrong in this sequence? AATCGCGUAACCGGCC

DNA is like a fingerprint because everyone’s is a little different! Revisit IBE DNA is like a fingerprint because everyone’s is a little different! You can tell people apart by their fingerprints……… and their DNA! How would the police look at DNA to figure out who committed a crime?

SWBAT explain how DNA replicates DNA replication Unit 5 LT 8.10.4b SWBAT explain how DNA replicates

http://www.youtube.com/watch?v=FR-du9JZqzQ

IBE In order for Peter Parker to become “Spiderman” what does it need to happen?

J-TPS (Justify –Think Pair Share)

DNA replication copies the genetic information of a cell.

During what stage of cell cycle does DNA get replicated?

During what stage of cell cycle does DNA get replicated?

DNA replication – helix unzips

DNA replication – two strands are separated

DNA replication – each side is now a template

DNA replication – two identical strands of DNA Original DNA strands

DNA replication Newly assembled DNA strands

DNA replication Semi-conservative replication

White board question DNA replication is A. the process of making proteins B. the process of making RNA C. the process of making new organelles D. the process of copying DNA

Proteins carry out the process of replication. nucleotide The DNA molecule unzips in both directions.

DNA Polymerase DNA polymerase brings nucleotides together to form the new DNA strand DNA polymerase new strand nucleotide

Semiconservative model of DNA The process of making new DNA molecules is semiconservative; one original and one new strand of DNA original strand new strand Two molecules of DNA

Leading strand synthesis continues in a 5’ to 3’ direction. Replication 5’ 3’ Overall direction of replication Leading strand synthesis continues in a 5’ to 3’ direction.

White board “The process of making new DNA molecules is semiconservative” What does it mean?

IBE In order for Peter Parker to become “Spiderman” what does it need to happen?

DNA replication video http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120076/micro04.swf::DNA%20Replication%20Fork