Antifungal activity of Momordica charantia seed extracts toward the pathogenic fungus Fusarium solani L. Shuzhen Wang, Yongliang Zheng, Fu Xiang, Shiming.

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Antifungal activity of Momordica charantia seed extracts toward the pathogenic fungus Fusarium solani L. Shuzhen Wang, Yongliang Zheng, Fu Xiang, Shiming Li, Guliang Yang Journal of Food and Drug Analysis Volume 24, Issue 4, Pages 881-887 (October 2016) DOI: 10.1016/j.jfda.2016.03.006 Copyright © 2016 Terms and Conditions

Figure 1 Growth inhibition of Fusarium solani on PDA plates. (A) a-1 to a-3, F. solani was cultured on the “control plate” for 12 hours, 24 hours, and 36 hours, respectively. (B) b-1 to b-3) F. solani incubated on the “treated plate” coated with MSE for 12 hours, 24 hours, and 36 hours, respectively. MSE=Momordica charantia seed extract; PDA=potato dextrose agar. Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions

Figure 2 Morphology observations of Fusarium solani hyphae under light microscope (×100). (A) Hyphae treated just with the protein extraction buffer. (B) The mycelia incubated with Momordica charantia seed extract (MSE). Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions

Figure 3 Microscopic observation of hyphae stained with propidium iodide (PI). “-1” and “-2”=the hyphae stained with PI was observed under the light microscope (×100); “-1-2” and “-2-2”=the PI stained hyphae was observed under the fluorescence microscopy (×100); “Con-”=the hyphae treated with protein extraction buffer, the healthy hyphae; “Tre-”=the seed extract treated hyphae, the unhealthy hyphae. Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions

Figure 4 Characterization of Fusarium solani genomic DNA on 3% agarose gel. Control=genomic DNA from the protein extraction buffer treated hyphae; M1 and M2=two different DNA markers; MSE=Momordica charantia seed extract; Treated=genomic DNA of MSE-treated hyphae, the black arrow indicating the short fragments. Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions

Figure 5 SDS-PAGE analysis (a) and Western blotting test of MSE with alpha-momorcharia antibody (b). Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions

Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10. 1016/j Journal of Food and Drug Analysis 2016 24, 881-887DOI: (10.1016/j.jfda.2016.03.006) Copyright © 2016 Terms and Conditions