Gel Documentation system(Gel Doc)

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Presentation transcript:

Gel Documentation system(Gel Doc)

Gel Documentation system Equipment widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended in agarose gels and polyacrylamide gels Other name: Gel image system or Gel imager Gel Documentation system has a specific light source so, before taking image of gel, it should be stained with fluorophores such as ethidium bromide or SYBR green which is matched to the light source of gel doc

Gel Documentation system Names of each part Strong camera basket F 1.0 8~48mm Manual lens ETBR filter Gel cutting switch Epi White light switch UV transliuminator switch

Gel Documentation system Names of each part UV safety for door open UV transiluminator UV switch

Specification Gel imager Camera Resolution 1.45 mega fixel Sensor CCD type The highest CCD sensor A+ Lens Control F 1.0 8~48mm Manual lens UV transiluminator Dual(306nm, 365nm) Gel cutting UV protection OK

Gel Documentation software Function of each button On/off button: close this software Softwareupdate: update this software Exposure: change the exposure time automatically or manually Saturation: get maximized band light Reverse: reverse the current image Acquire: capture the current image 3 4 5 6 1 2

Gel Documentation software Function of each button Annotation: input arrow, text or date Reverse: Reverse image of captured Spot zoom: Image zoom Histogram: Image control Gel cutting: change to gel cutting mode Save: Save the current image Print: Print out image Back: move to before window 6 7 8 1 2 3 4 5

How to use After agarose gel electrophoresis 1 2 3 4 5 Turn on the gel imager Open the door Put agarose gel on the UV transiluminator and turn on the UV Close the door and acquire the image Save the image