Proliferation of Mature Ex Vivo Human Dental Pulp Using Tissue Engineering Scaffolds  Sheila Chandrahasa, DMD, Peter E. Murray, PhD, Kenneth N. Namerow, DDS  Journal of Endodontics  Volume 37, Issue 9, Pages 1236-1239 (September 2011) DOI: 10.1016/j.joen.2011.05.030 Copyright © 2011 Terms and Conditions

Figure 1 Histology of metabolically active cells from mature human dental pulp that proliferated into (A) polymer, (B) collagen, and (C) calcium phosphate tissue engineering scaffolds. The cells were observed proliferating into the tissue engineering scaffolds after 30 days of culture in contact with the dental pulp from mature tooth slices. The metabolically active cells were stained with neutral-red dye. The larger areas of dark staining are clusters of proliferating cells. Journal of Endodontics 2011 37, 1236-1239DOI: (10.1016/j.joen.2011.05.030) Copyright © 2011 Terms and Conditions

Figure 2 Histology of stem cells from human exfoliated deciduous teeth that proliferated into (A) polymer, (B) collagen, and (C) calcium phosphate tissue engineering scaffolds after 30 days of culture. The metabolically active cells were stained with neutral-red dye. The larger areas of dark staining are clusters of proliferating cells. Journal of Endodontics 2011 37, 1236-1239DOI: (10.1016/j.joen.2011.05.030) Copyright © 2011 Terms and Conditions

Figure 3 Proliferation of mature human dental pulp into tissue engineering scaffolds. Calcium phosphate scaffolds: average rate of cell proliferation: 1.305 mature dental pulp cells per day (R2 = .549), collagen scaffolds: average rate of cell proliferation: 7.195 cells per day (R2 = .910), and polymer scaffolds: average rate of cell proliferation: 13.885 cells per day (R2 = .868). R2 is the coefficient of linear regression correlation between the data and the average rate of cell proliferation. Journal of Endodontics 2011 37, 1236-1239DOI: (10.1016/j.joen.2011.05.030) Copyright © 2011 Terms and Conditions