Morphological Characters ISOLATION OF MULTIPLE DRUG RESISTANT Citobacter sp. AR2 FROM GANGES WATER Atanu Roy1*, Anuradha Banerjee1, Diganta Sarkar1, Prateeti Basu2, Rima Roy2 and Madhumita Roy1 1Department of Biotechnology, Techno India University, Salt Lake Campus Kolkata 700091. 2Department of Microbiology, Techno India University, Salt Lake Campus, Kolkata 700091. Introduction In 2007 River Ganga has been declared as the fifth most polluted river in the world1,2. Dumping of industrial and municipal sewage has made the river water a breeding ground for multiple antibiotic resistant coliforms 3,4. This present study was conducted to study the presence of multi drug resistant coliforms in Ganges water collected from two heavily populated ghats of Kolkata and Howrah: Dakhinweswar and Belur. Results and Discussion Growth curve of Citrobacter sp.AR2  Morphological Characters Citrobacter sp. AR2 Colour of colony Creamish Cell shape Rod Gram staining Negative Motility Motile PCR product of 16s rDNA. Accession number is KU362710.1 Sl. No. Antibiotics Citrobacter sp. AR2 A.    Hexa G Plus 6 1 Ampicillin R 2 Chloramphenicol 3 Penicillin G 4 Streptomycin 0.85 5 Sulphatriad 6 Tetracycline 0.9 B.     Hexa G Minus 2 7 Ceftazidime 0.98 8 Ciprofloxacin 9 Amikacin 1.6 10 Nitrofurantoin 11 Netillin 1.8 12 Nalidixic acid C.    Hexa G Minus 3 13 Co-Trimoxazole 14 Amoxyclav 15 Gentamicin 1.4 16 17 Ofloxacin 18 Cefuroxime D.    Hexa G Minus 5 19 Cefotaxime 1.1 20 Levofloxacin 21 Aztreonam 1.2 22 Imipenem 23 1.5 24 E.    Hexa G Minus 1 25 26 27 28 29 30 Tobramycin Change in pH due to growth of the organism Future Work Plasmid mediated drug resistance Bioremediation properties Materials and Methods Samples were collected Plated with different antibiotics by pour plate method Tested against commercially available antibiotic discs Biochemical tests performed 16s rDNA analysis was performed Growth curve, MIC for major antibiotics performed Acknowledgements The authors would like to express their deepest gratitude to Techno India University for providing all the facilities required for this research work. They would also like to give a vote of thanks to all the faculties and scholars of Department of Biotechnology and Microbiology for their support and help all through the work. Reference 1.  Ghosh, A., 17 October 2012. "Ganga is now a deadly source of cancer, study says",  http://articles.timesofindia.indiatimes.com/2012-10-18/patna/ 34554229_ 1_gall-bladder-cancer-cancer-patients-prostate.   2. CENTRAL POLLUTION CONTROL BOARD (Ministry of Environment and Forests), Govt. of India July 2013. Pollution assessment: River Ganga. 3. Levy, SB. 1998. The challenge of antibiotic resistance. Sci Am. 278, 46-53. 4. O’Grady F, Lambert HP, Finch RG, Greenwood D. 1997. Antibiotics and chemotherapy. 7th ed. New York: Churchill Livingstone. Table demonstrate the multidrug resistance ability of Citrobacter sp. against different antibiotics