anti-hnRNP A2 (U/mL) anti-hnRNP B1 (U/mL)

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1 10 100 1000 anti-hnRNP A2 (U/mL) anti-hnRNP B1 (U/mL) Supplementary Figure 1: Comparison of anti-hnRNP A2 and anti-hnRNP antibody analyis: Testing 299 sera of patients suffering from rheumatoid arthritis, anti-hnRNP B1 antibody analysis by the novel ELISA was correlated with anti-hnRNP A2 (RA33) antibody detection by a commercially available ELISA (HUMAN, Wiesbaden, Germany) (Spearman’s rho = 0.209, 95% confidential interval: 0.098 – 0.315, p < 0.001).

C – control serum (blood donor) 55 kDa – 43 kDa – 30 kDa – M 1 2 3 4 5 6 7 8 9 10 P C Supplementary Figure 2: Immunoblot analysis of anti-hnRNP B1 antibody reactivity: Five sera of patients suffering from rheumatoid arthritis (1 – 5) and 5 sera of patients with systemic sclerosis (5 – 10) were tested for IgG reactivity to recombinant hnRNP B1 (in.vent DIAGNOSTICA GmbH, Hennigsdorf, Germany) blotted onto a nitrocellulose membrane after SDS polyacrylamide gel eletrophoresis. C – control serum (blood donor) P – Ponceau staining of the blotted hnRNP B1 polypeptide