From: Impact of Vital Dyes on Cell Viability and Transduction Efficiency of AAV Vectors Used in Retinal Gene Therapy Surgery: An In Vitro and In Vivo Analysis.

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From: Impact of Vital Dyes on Cell Viability and Transduction Efficiency of AAV Vectors Used in Retinal Gene Therapy Surgery: An In Vitro and In Vivo Analysis Trans. Vis. Sci. Tech.. 2017;6(4):4. doi:10.1167/tvst.6.4.4 Figure Legend: Effect of DB dilutions and of single dye components on AAV titer assessed by qPCR. DB was used neat and at two dilutions (1:50 and 1:125) to prepare an AAV solution that was titered by qPCR and normalized to no dye sample. The effects of three dye components (trypan blue, PEG, and PF-68) were tested in parallel; PF-68 was also tested in combination with DB diluted 1:50. Values are mean of six replicates ± SEM. Both neat and 1:50 diluted DB increased apparent AAV titer compared with no dye samples (one-way ANOVA, with Dunnett's multiple comparison test; ***P < 0.001; ****P < 0.0001). Of all three components tested, PEG was the only component that induced a significant increase in recorded qPCR titer (one-way ANOVA, with Dunnett's multiple comparison test; *P = 0.0403). Date of download: 11/15/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.