Copyright © 2015 American Medical Association. All rights reserved. From: Clinical, Histologic, and Molecular Analysis of Differences Between Erythematotelangiectatic Rosacea and Telangiectatic Photoaging JAMA Dermatol. 2015;151(8):825-836. doi:10.1001/jamadermatol.2014.4728 Figure Legend: Clinical Photographs of Erythematotelangiectatic Rosacea (ETR) and Telangiectatic Photoaging (TP)A-D, Facial telangiectasia and erythema tended to be located on the central face in ETR. E-H, Telangiectasia and erythema tended to be located on the lateral face in TP. A and E, Circles show biopsy locations; in both conditions, biopsies were centered on a dilated vessel. B-D and F-H, Enlargements show clinical features of the central face (B and F), preauricular area (C and G), and jawline (D and H). Date of download: 11/15/2017 Copyright © 2015 American Medical Association. All rights reserved.

Copyright © 2015 American Medical Association. All rights reserved. From: Clinical, Histologic, and Molecular Analysis of Differences Between Erythematotelangiectatic Rosacea and Telangiectatic Photoaging JAMA Dermatol. 2015;151(8):825-836. doi:10.1001/jamadermatol.2014.4728 Figure Legend: Histopathologic Findings in Erythematotelangiectatic Rosacea (ETR), Telangiectatic Photoaging (TP), and Control SpecimensThe x-axis numbers 0 through 4 indicate degrees of severity on a scale explained in detail for each domain in eTable 1 in the Supplement. Briefly, 0 indicates a normal, healthy state in all domains; 4 indicates the most severe condition in the specified domain. Date of download: 11/15/2017 Copyright © 2015 American Medical Association. All rights reserved.

Copyright © 2015 American Medical Association. All rights reserved. From: Clinical, Histologic, and Molecular Analysis of Differences Between Erythematotelangiectatic Rosacea and Telangiectatic Photoaging JAMA Dermatol. 2015;151(8):825-836. doi:10.1001/jamadermatol.2014.4728 Figure Legend: Histopathologic Features of Erythematotelangiectatic Rosacea (ETR), Telangiectatic Photoaging (TP), and Control SpecimensA, Comparison of characteristic light microscopic features (top; hematoxylin-eosin) and transmission electron micrographic features (bottom). Under light microscopy, the ETR specimen has dilated vessels surrounded by intact collagen (green asterisks) and a greater degree of inflammation than the TP specimen; TP specimen has dilated vessels surrounded by more fragmented collagen (black asterisks) and a greater degree of solar elastosis (yellow asterisks). Under electron microscopy, the ETR specimen shows dilated blood vessel with multiple intact adjacent collagen fibrils (inset shows crosscut collagen, original magnification ×400); the TP specimen shows dilated blood vessel surrounded by areas of unstructured, damaged fibers (inset, original magnification ×400) and some amorphous material consistent with elastotic material (yellow asterisk). B, Light microscopic features of mast cells (mast cell tryptase stain). The control specimen shows stain localized to mast cells (inset, original magnification ×400). The ETR specimen shows staining of mast cells in addition to light brown staining of the area surrounding mast cells, indicative of mast cell degranulation (inset, original magnification ×400). C, Immunohistochemical quantitative image analysis of mast cell tryptase staining; area demonstrated greater than 4-fold increased staining in ETR vs TP, and 25-fold greater staining in ETR vs control. There was 6-fold increased staining in TP vs control. Date of download: 11/15/2017 Copyright © 2015 American Medical Association. All rights reserved.

Copyright © 2015 American Medical Association. All rights reserved. From: Clinical, Histologic, and Molecular Analysis of Differences Between Erythematotelangiectatic Rosacea and Telangiectatic Photoaging JAMA Dermatol. 2015;151(8):825-836. doi:10.1001/jamadermatol.2014.4728 Figure Legend: Number-Fold Induction Changes of Gene Transcripts Relevant to Rosacea as Determined by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) in Erythematotelangiectatic Rosacea (ETR), Telangiectatic Photoaging (TP), and Control (Ctrl) SpecimensA-E, In all panels, asterisks indicate P < .05, and error bars, standard errors. A, For neuropeptide gene transcripts, RT-PCR showed induction of gene expression of calcitonin gene-related peptides (CGRP)-α (CALCA), CGRP-β (CALCB), and substance P (TAC1) in ETR compared with Ctrl, and upregulation of CGRP-α and substance P compared with TP. B, For collagen gene transcripts, RT-PCR showed upregulated gene expression of types I (COL-1) and III (COL-3) collagen in ETR compared with Ctrl and TP. C, For matrix remodeling gene transcripts decorin (DCN) and Cyr-61 (CYR61), RT-PCR revealed upregulated gene expression in ETR compared with Ctrl and TP. D, For matrix metalloproteinases (MMPs)-1, 3, and 9 gene transcripts, RT-PCR showed upregulation of all 3 MMPs in ETR compared with Ctrl; MMP-3 was also upregulated compared with TP; MMP-1 and MMP-9 were upregulated in TP compared with Ctrl. E, For gene transcripts involved in mast cell chemotaxis, RT-PCR showed upregulation of chemokine CXCL12 and its receptor, CXCR4, in ETR compared with Ctrl; CXCR4 was also upregulated in ETR compared with TP. Date of download: 11/15/2017 Copyright © 2015 American Medical Association. All rights reserved.