Improved fat transplantation survival by using the conditioned medium of vascular endothelial growth factor transfected human adipose-derived stem cells

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Improved fat transplantation survival by using the conditioned medium of vascular endothelial growth factor transfected human adipose-derived stem cells Yang Zhang, Li-Ling Xiao, Jiang-Xuan Li, Hong-Wei Liu, Sheng-Hong Li, Yan-Yun Wu, Xuan Liao, Cong-Qiang Rao The Kaohsiung Journal of Medical Sciences Volume 33, Issue 8, Pages 379-384 (August 2017) DOI: 10.1016/j.kjms.2017.05.009 Copyright © 2017 Kaohsiung Medical University Terms and Conditions

Fig. 1 The fluorescence intensity and the expression of VEGF of the CM in different group. (A) The fluorescence intensity in MOI = 0, 10, 20, 30, 40, 50 in different time point. (magnification ×200). (B) The expression of VEGF in MOI = 0, 10, 20, 30, 40, 50 in different time point. *P < .05; **P < .01; ns, no significance (Student–Newman–Keuls test). The Kaohsiung Journal of Medical Sciences 2017 33, 379-384DOI: (10.1016/j.kjms.2017.05.009) Copyright © 2017 Kaohsiung Medical University Terms and Conditions

Fig. 2 Comparison of the size of fat residues in the four groups on day 4, day 7, day 15, day 30, day 60. The Kaohsiung Journal of Medical Sciences 2017 33, 379-384DOI: (10.1016/j.kjms.2017.05.009) Copyright © 2017 Kaohsiung Medical University Terms and Conditions

Fig. 3 Fat grafts were stained with hematoxylin and eosin (magnification ×200). The Kaohsiung Journal of Medical Sciences 2017 33, 379-384DOI: (10.1016/j.kjms.2017.05.009) Copyright © 2017 Kaohsiung Medical University Terms and Conditions

Fig. 4 (A) The expression of VEGF by ELISA; (B) the expression of VEGF by western blot; (C) quantitative analysis of protein levels by densitometry.*P < .05; **P < .01; ns, no significance (Student–Newman–Keuls test). The Kaohsiung Journal of Medical Sciences 2017 33, 379-384DOI: (10.1016/j.kjms.2017.05.009) Copyright © 2017 Kaohsiung Medical University Terms and Conditions