Exosome-enclosed microRNAs in exhaled breath hold potential for biomarker discovery in patients with pulmonary diseases Anirban Sinha, MSc, Amit Kumar.

Slides:

Advertisements

Similar presentations

Genome-wide association study identifies TH1 pathway genes associated with lung function in asthmatic patients Xingnan Li, PhD, MS, Gregory A. Hawkins,

Advertisements

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Genome-wide association study identifies TH1 pathway genes associated with lung function in asthmatic patients Xingnan Li, PhD, MS, Gregory A. Hawkins,

Mauro Maniscalco, MD, PhD, Debora Paris, PhD, Dominique J

Asthma inflammatory phenotypes show differential microRNA expression in sputum Tania Maes, PhD, Francisco Avila Cobos, MSc, Florence Schleich, MD, PhD,

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,

Ryan H. Dougherty, MD, Sukhvinder S

Epithelial proteome profiling suggests the essential role of interferon-inducible proteins in patients with allergic rhinitis Joseph Ndika, PhD, Liisa.

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

Dissecting childhood asthma with nasal transcriptomics distinguishes subphenotypes of disease Alex Poole, MS, Cydney Urbanek, BS, Celeste Eng, BS, Jeoffrey.

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD

Is 9 more than 2 also in allergic airway inflammation?

Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma Ronaldo P. Panganiban, BS, Yanli Wang, BS, Judie Howrylak, MD, PhD,

Toll-like receptor 4 ligation enforces tolerogenic properties of oral mucosal Langerhans cells Jean-Pierre Allam, MD, Wen-Ming Peng, MSc, Torsten Appel,

Gender differences in the bronchoalveolar lavage cell proteome of patients with chronic obstructive pulmonary disease Maxie Kohler, PhD, AnnSofi Sandberg,

Proinflammatory role of epithelial cell–derived exosomes in allergic airway inflammation Ankur Kulshreshtha, MTech, Tanveer Ahmad, MSc, Anurag Agrawal,

Severe atopic dermatitis is characterized by selective expansion of circulating TH2/TC2 and TH22/TC22, but not TH17/TC17, cells within the skin-homing.

Blood DNA methylation biomarkers predict clinical reactivity in food-sensitized infants David Martino, PhD, Thanh Dang, PhD, Alexandra Sexton-Oates, BSc,

A critical role for IL-18 in transformation and maturation of naive eosinophils to pathogenic eosinophils Sathisha Upparahalli Venkateshaiah, PhD, Akanksha.

Serum from Asian patients with atopic dermatitis is characterized by TH2/TH22 activation, which is highly correlated with nonlesional skin measures Huei-Chi.

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Small RNA profiling reveals deregulated phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/Akt pathway in bronchial smooth muscle.

Comparative transcriptomic analyses of atopic dermatitis and psoriasis reveal shared neutrophilic inflammation David F. Choy, BS, Daniel K. Hsu, PhD,

An integrated model of alopecia areata biomarkers highlights both TH1 and TH2 upregulation Teresa Song, BS, Ana B. Pavel, PhD, Huei-Chi Wen, MD, PhD,

Yuichi Teraki, MD, Aika Sakurai, MD, Seiichi Izaki, MD

Jerome A. Sigua, MD, Becky Buelow, MD, Dorothy S

Lower airway microbiota and mycobiota in children with severe asthma

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Airway epithelial cells from asthmatic children differentially express proremodeling factors Jesus M. Lopez-Guisa, PhD, Claire Powers, BA, Daniele File,

Ten-eleven translocation 1 (TET1) methylation is associated with childhood asthma and traffic-related air pollution Hari K. Somineni, MS, Xue Zhang,

A network-based analysis of the late-phase reaction of the skin

Peter M. Wolfgram, MD, David B. Allen, MD

Profiling of human CD4+ T-cell subsets identifies the TH2-specific noncoding RNA GATA3-AS1 Huan Zhang, MD, PhD, Colm E. Nestor, PhD, Shuli Zhao, PhD,

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Pulmonary sarcoidosis is associated with exosomal vitamin D–binding protein and inflammatory molecules Maria-Jose Martinez-Bravo, PhD, Casper J.E. Wahlund,

Fibronectin is a TH1-specific molecule in human subjects

Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses Stephan Traidl, Petra Kienlin,

Cigarette smoke combined with Toll-like receptor 3 signaling triggers exaggerated epithelial regulated upon activation, normal T-cell expressed and secreted/CCL5.

Suppression of the basophil response to allergen during treatment with omalizumab is dependent on 2 competing factors Donald W. MacGlashan, MD, PhD,

Jon Genuneit, MD, MSc Journal of Allergy and Clinical Immunology

Nasal epithelium as a proxy for bronchial epithelium for smoking-induced gene expression and expression Quantitative Trait Loci Kai Imkamp, MD, Marijn.

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Expression of programmed cell death ligand-1 in mastocytosis correlates with disease severity Anja Rabenhorst, PhD, Silke Leja, MTA, Juliana Schwaab,

Statins enhance the anti-inflammatory effects of inhaled corticosteroids in asthmatic patients through increased induction of indoleamine 2, 3-dioxygenase

Atopic dermatitis: A practice parameter update 2012

Ten-eleven translocation 1 (TET1) methylation is associated with childhood asthma and traffic-related air pollution Hari K. Somineni, MS, Xue Zhang,

Different IgE recognition of mite allergen components in asthmatic and nonasthmatic children Yvonne Resch, MSc, Sven Michel, MSc, Michael Kabesch, MD,

IL-9 is a key component of memory TH cell peanut-specific responses from children with peanut allergy Helen A. Brough, MSc, FRCPCH, David J. Cousins,

Autophagy: Nobel Prize 2016 and allergy and asthma research

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Effect of aging on sputum inflammation and asthma control

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells Véronique Schulten, PhD, Victoria Tripple, BSc, Grégory Seumois,

Correlation between CCL26 production by human bronchial epithelial cells and airway eosinophils: Involvement in patients with severe eosinophilic asthma

Baochen Shi, PhD, Nathanael J

Tertiary lymphoid organs: A novel target in patients with chronic rhinosinusitis Sathish Paramasivan, MBBS, BMedSci(Hons), MPH, Susan Lester, BSc(Hons),

Exosome-enclosed microRNAs in exhaled breath hold potential for biomarker discovery in patients with pulmonary diseases Anirban Sinha, MSc, Amit Kumar.

Genome-wide association studies of asthma indicate opposite immunopathogenesis direction from autoimmune diseases Xingnan Li, PhD, Elizabeth J. Ampleford,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Differential expression of microRNAs in exhaled breath condensates of patients with asthma, patients with chronic obstructive pulmonary disease, and healthy.

Karl J. Staples, PhD, Timothy S. C. Hinks, MB BS, Jon A

Large-scale gene expression profiling reveals distinct type 2 inflammatory patterns in chronic rhinosinusitis subtypes Matthew A. Tyler, MD, Chris B.

Statins enhance the anti-inflammatory effects of inhaled corticosteroids in asthmatic patients through increased induction of indoleamine 2, 3-dioxygenase

Aarti Shikotra, BSc, David F. Choy, BSc, Chandra M

Epigenetic mechanisms and the development of asthma

Presentation transcript:

Exosome-enclosed microRNAs in exhaled breath hold potential for biomarker discovery in patients with pulmonary diseases Anirban Sinha, MSc, Amit Kumar Yadav, MSc, Samarpana Chakraborty, MSc, S.K. Kabra, MD, R. Lodha, MD, Manish Kumar, MSc, Ankur Kulshreshtha, MTech, Tavpritesh Sethi, MBBS, Rajesh Pandey, PhD, Gaurav Malik, BTech, Saurabh Laddha, MSc, Arijit Mukhopadhyay, PhD, Debasis Dash, PhD, Balaram Ghosh, PhD, Anurag Agrawal, MD, PhD Journal of Allergy and Clinical Immunology Volume 132, Issue 1, Pages 219-222.e7 (July 2013) DOI: 10.1016/j.jaci.2013.03.035 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 EBC miRNAs are potential biomarkers and contained in exosomes. A, Correlation bubble plot of miRNA expression between the healthy and asthma groups. Color (red to blue) and size (small to large) denote sample counts in the asthma and healthy groups, respectively. miRNAs with high sample counts (large blue circles) were selected from the regions away from the unity line with a fold change of 2 or greater (axes truncated; see the extended legend for Fig 1 in this article's Online Repository at www.jacionline.org for details). B, Scatter plot of miRNA expression between the asthma and tuberculosis groups. Red and blue circles indicate miRNAs overexpressed and underexpressed in asthmatic patients with respect to healthy subjects, respectively. Black circles indicate miRNAs with no differential expression in the 3 groups. C, Fluorescence of CD9/MHC-II measured by means of fluorescence-activated cell sorting confirming that EBC contains exosomes. CellSup, Cell culture supernatant (positive control). PBS is the negative saline control. D, Relative miRNA content of the exosome (CD63 pellet) and nonexosome (supernatant), confirming that exosomes contain the majority of miRNAs. Journal of Allergy and Clinical Immunology 2013 132, 219-222.e7DOI: (10.1016/j.jaci.2013.03.035) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Heat map showing differential expression of genes (x-axis, column) in nasal scrapings across 6 healthy subjects (N) and 21 asthmatic patients (A). The gene expression values were rescaled to lie between an arbitrarily selected range of 0 to 1. The numeric values were shown as colors indicating the relative expression of genes, with red being the most expressed and green being the least expressed. Journal of Allergy and Clinical Immunology 2013 132, 219-222.e7DOI: (10.1016/j.jaci.2013.03.035) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 A, The forward scatter (FSC)–side scatter (SSC) plot shows the bead selection for detection of bead-bound exosomes. The selected region marks the region for further analysis across various groups. B, Exosomal marker proteins (Tsg-101 and Alix) are seen in anti-CD63 antibody–coated beads in comparison with uncoated beads. Journal of Allergy and Clinical Immunology 2013 132, 219-222.e7DOI: (10.1016/j.jaci.2013.03.035) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 The most significant pathways reflected from the predicted gene list of the significant miRNAs using DAVID. Journal of Allergy and Clinical Immunology 2013 132, 219-222.e7DOI: (10.1016/j.jaci.2013.03.035) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Asthma is reflected as one of the most important pathways from the differentially regulated epithelial genes in asthmatic patients and healthy subjects. Journal of Allergy and Clinical Immunology 2013 132, 219-222.e7DOI: (10.1016/j.jaci.2013.03.035) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions