Volume 55, Issue 3, Pages 659-668 (March 2009) Microsatellite Analysis of Voided-Urine Samples for Surveillance of Low-Grade Non- Muscle-Invasive Urothelial Carcinoma: Feasibility and Clinical Utility in a Prospective Multicenter Study (Cost-Effectiveness of Follow-Up of Urinary Bladder Cancer Trial [CEFUB])  Madelon N.M. van der Aa, Ellen C. Zwarthoff, Ewout W. Steyerberg, Merel W. Boogaard, Yvette Nijsen, Kirstin A. van der Keur, Antonius J.A. van Exsel, Wim J. Kirkels, Chris Bangma, Theo H. van der Kwast  European Urology  Volume 55, Issue 3, Pages 659-668 (March 2009) DOI: 10.1016/j.eururo.2008.05.001 Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 1 Prolonged duration between urine collection and microsatellite analysis (MA) and intravesical therapy negatively affect quality of MA in 19% (197/1012) of the samples. (a) Effect of time until analysis of urine sample. After 2 wk of delay MA failed in 80%. (b) Effect of intravesical immune/chemotherapy (iv therapy). In the first 6 mo after intravesical therapy, MA of 15% of urine samples failed, compared with 8% in samples from patients without iv therapy. A total of 88 patients was treated with intravesical therapy after transurethral resection. European Urology 2009 55, 659-668DOI: (10.1016/j.eururo.2008.05.001) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 2 Flowchart showing the possible endpoints after completion of a microsatellite analysis (MA) series and the accompanying numbers of events. Endpoints were final (end of study or progressive recurrence) or temporary (change of MA result or urothelial carcinoma [NMI-UC]). Temporary endpoints and series ending with a histologically proven non-muscle invasive recurrence restarted a new MA series (*). European Urology 2009 55, 659-668DOI: (10.1016/j.eururo.2008.05.001) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 3 Kaplan-Meier curve shows the proportion of recurrences in 458 microsatellite analysis (MA) events detected by consecutive continuous positive or negative results of MA. Time (in months) reflects the length of time of continuous MA results without visible suspicious lesion in the bladder. After 24 mo, 83% of recurrences were detected; 22% were missed. European Urology 2009 55, 659-668DOI: (10.1016/j.eururo.2008.05.001) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 4 Kaplan-Meier curves relating fibroblast growth factor receptor 3 (FGFR3) gene mutation status and tumour multifocality at entry to microsatellite analysis-detected recurrences. European Urology 2009 55, 659-668DOI: (10.1016/j.eururo.2008.05.001) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 5 Kaplan-Meier curves relating gender and smoking habits demonstrate that smoking habits determine the proportion of microsatellite analysis-detectable recurrences. European Urology 2009 55, 659-668DOI: (10.1016/j.eururo.2008.05.001) Copyright © 2008 European Association of Urology Terms and Conditions