From: Volatile Anesthetics Bind Rat Synaptic Snare Proteins Anesthes. 2005;103(4):768-778. Figure Legend: *P  < 0.0001  versus  PBS T2. (  G  ) Western blot with syntaxin antibody of fast protein liquid chromatography buffer purified SNARE complex to isolate low- and high-MW species. Peak fractions eluted from a shallow ionic strength gradient were analyzed by PAGE for SNARE complex size. Fractions containing primarily low-MW or high-MW SNARE complex were pooled, dialyzed against PBS, and separated on PAGE before staining with a syntaxin antibody. Migration of proteins of known molecular weight are shown to the  left. Lanes  from  left  to  right  : 1: unboiled low-MW SNARE complex pooled fractions; 2: boiled; 3: unboiled high-MW SNARE complex; 4: boiled; 5: syntaxin monomer used for SNARE complex formation. (  H  ) Spin-spin relaxation curves for the low- and high-MW SNARE complexes in  G  . T2Low MW= 0.682 ± 0.022; T2High MW= 0.532 ± 0.021; [Isoflurane]= 0.87 mm; [Low-MW SNARE complex]= 11.9 μm; [High-MW SNARE complex]= 10.7 μm. T2Low MW> T2High MW,  P  < 0.001 by nonlinear regression, F1,28= 1,010; T2Low MWand T2High MW< T2PBS,  P  < 0.001, F1,28(Low MW  vs.  PBS) = 445.2, F1,28(High MW  vs.  PBS) = 1,566.  Date of download: 11/22/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.