Intermediate and High Voltage Microscopy

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Presentation transcript:

Intermediate and High Voltage Microscopy د. عمر عبد القادر

د. عمر عبد القادر

The greater the accelerating voltage the shorter the l Therefore, a 50,000 volt (50 kV) electron has a wavelength of 0.0055nm and a 1MeV electron has a wavelength of 0.00123nm! د. عمر عبد القادر

A million volt (MeV) TEM must maintain an accelerating voltage that is stable to within 0.5 volts. د. عمر عبد القادر

A million volt (MeV) TEM must maintain an accelerating voltage that is stable to within 0.5 volts. With this coupled with a field emission source one can achieve resolutions that are in the range of 0.5Å د. عمر عبد القادر

Hitachi 3 MeV is the most powerful TEM ever made د. عمر عبد القادر

Operator Hitachi 3 MeV is the most powerful TEM ever made د. عمر عبد القادر

It requires its own separate building د. عمر عبد القادر

Advantages of High Voltage EM Increased Resolution: Although still a long way from a theoretical resolution of 0.0006 nm. the best actual resolution has been achieved with a 1 MeV field emission TEM. Nanocrystalline structure of silicon د. عمر عبد القادر

Advantages of High Voltage EM Increased Resolution: Although still a long way from a theoretical resolution of 0.0006 nm. the best actual resolution has been achieved with a 1 MeV field emission TEM. 1 MeV 200 kV 100kV د. عمر عبد القادر

Advantages of High Voltage EM Increased Specimen Penetration: As accelerating voltage increases the ability of the beam to penetrate also increases. د. عمر عبد القادر

Dendrite in 3 mm thick section Advantages of High Voltage EM Increased Depth of Information: The great depth of information allows an entire thick section to be viewed at once. Dendrite in 3 mm thick section د. عمر عبد القادر

Mitotic cell stereo pair Advantages of High Voltage EM Increased Depth of Information: It also allows for stereo pairs to be created from thick sections. Mitotic cell stereo pair د. عمر عبد القادر

Advantages of High Voltage EM Reduced Beam Damage: The increased speed of the electrons actually decreases the likelihood of an inelastic collision. The same section viewed with 1 MeV vs. 100 kV will have less contrast due to fewer scattered electrons د. عمر عبد القادر

The Boulder Laboratory for 3-Dimensional Electron Microscopy of Cells is located at the University of Colorado. It is a National Resource Center supported by the National Center for Research Resources (NCRR) at the National Institutes of Health (NIH). د. عمر عبد القادر

Intermediate Voltage (200-400 kV) JEOL JEM-4000FX Hitachi H-9000NAR د. عمر عبد القادر