Varian 940-LC Analytical to Prep HPLC

Varian 940-LC Silver Bullets The 940-LC one instrument – many uses: High performance automated Analytical HPLC High performance semi-preparative HPLC High performance prep LC

Varian 940-LC Silver Bullets Flexible and capable Wide flow rate from 0.1 ml/min to 200 ml/min Dual path-length flowcells for extended Abs One Injector, analytical or prep volumes (5ul to 4.5ml) Superb fraction collecting Galaxie real-time peak detection Practical collect and inject Much Easier interface, it does what you tell it! Cleaner UI

Goals of the 940-LC Analytical to Prep Aim Become market leader in this $180M segment Double market share Market Continue to focus on Pharma / BioPharma Academic Strategy Deliver innovation in hardware, consumables, software and services

Varian 940-LC Instrument Overview Factory tested, pre-plumbed instrument: Plug and play operation High performance semi-preparative HPLC Gradient Analytical HPLC Autoprep System Choice of UV or PDA detector Dual path flow cell for unmatched range and scalability No compromise performance for prep and analytical – switch with a key stroke – no plumbing or hardware changes needed! The most flexible analytical to prep HPLC available Fast prep Integ fc co;\llection Perform Consumables Ana to skid

Varian 940-LC Instrument Overview Scale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep work Versatile and robust solvent-delivery system New loopless autosampler – wide volume range NEW Fraction collection with Complete range of collection modes such as slope, threshold and time slicing Wide range of vessels, including autosampler rack Comprehensive range of columns and consumables More productivity via GalaxieTM software Valve automation for genuine unattended operation NEW direct control interface

Varian 940-LC Versatility and Performance 1 ml/min 21 ml/min Easy to change pump heads Versatile solvent delivery systems Powerful pumps allow 4000psi at 25mL/min operation for fast chromatography Ideal for 4.6mm and 1” column Able to use smaller 3 & 5 um particles columns at elevated pressures Improves productivity Save solvents and waste costs Short run times save time High resolution separations = high purity We have uncovered the market need -

Varian 940-LC Pumps Same range of accessories as PS210/218 5ml Head to 200ml Head Easily interchangeable pressure modules thru removable side panel. Easy access to mixer Self maintenance and versatility features have been completely retained with no compromises – integrated systems are notoriously difficult to maintain so this is Unique

Varian 940-LC Pumps - FAB Feature Advantage Benefit Interchangeable Pump Heads (5 to 200ml/min) Unrivalled flexibility for any application Economical and Convenient with Excellent Performance Open access for ease of maintenance Easy for the user to correct basic issues resulting in more uptime Dependable Simple and system Durability Analytical to Prep on one System One system will do the work of two providing high performance flexibility Performance and Reliability at an Excellent Price/Performance ratio

Varian 940-LC Autosampler Prep version of the 920-LC Sampler 5ul to 4.5ml Injection range No necessity to combine a Manual injector No need to dedicate injector to prep or analytical One Sampler does all! Cooled version Loopless Design Needle in path Design No carryover 2008 Technology – no changing loops

Varian 940-LC Autosampler - FAB Feature Advantage Benefit Needle in Path Design No need for Injection loops No Carryover Versatility, Economical Performance One Injector, large or small inject volumes Simplifies the system, no need to swap components for analytical to prep Accuracy and Seamless Simplicity One Autosampler, all rack formats No need for multiple injector options Versatility Simplicity

Varian 940-LC UV/Vis - PDA Detectors High Sensitivity for Analytical applications Extended absorbance – desensitised, for Prep Applications All in One Cell! NEW 4 x 0.25mm Cell low dead volume Excellent performance at low flow rates for Analytical Excellent Extended Absorbance for Prep

Varian 940-LC Dual Pathlength Flowcell Absorbance Detector Extended absorbance range without re-plumbing (<70 AU) NEW 4mm x 0.25 mm 4mm x 0.15 SuperPrep Low dispersion UV/Vis Detector PDA Detector Low noise ~ +10 mAU Low Drift ~ 1 mAU/hr

Agilent 1200 Flowcells

Varian 940-LC UV/Vis - PDA Detectors - FAB Feature Advantage Benefit NEW 4x0.25 Low Volume Cell Optimised for Analytical to Prep, no need to change Cells Performance and Versatility and Dependable Tool Free maintenance Easy to change lamps and flow cells Simplicity Range of cells for ul/min to Litres/min Fits all applications from Analytical to Benchtop Prep Versatility and Performance

Varian 440-LC Fraction Collector Fully controlled by software for foolproof collection by slope, level or timeslice Known fluid path for accurate fraction collection Software display of collected fractions and source injections Reliable operation Compatible with a wide range of containers Belart and other optional racks Microvial to 50ml tube capacity Autosampler rack compatibility provides practical collect and re-inject capabilities

Varian 440-LC Fraction Collector We chose not to collect Peak 3 Peaks 1,2,4,5,6,7 collect with exceptional accuracy Excellent algorithm, and simple delay volume calculation Slope/Threshold based on real numbers!!

Varian 440-LC Fraction Collector Fraction Log shows peak location using colour coded rack assignment

Varian 440-LC Fraction Collector - FAB Feature Advantage Benefit Modern Design, improved movement and hardware Significant improvement ergonomically on 701 Pleasing Appearance with Stylish fast Collector movement Simplified user interface Easy to use, does what you ask it to do both manually and automated Simplicity and precious Dependability for customer fractions Inject/Collect and Re-inject Collect into A/sampler rack then re-insert into the Sampler for Fraction Purity Determination Provides Confidence in Fraction Puritys without the need to do any liquid transfers

Varian 445-LC Scale-up Module Optimized flow paths for automated scale-up Separate optimised flow paths for Prep and Analytical columns Solvent selection valves for 4 solvents ( 2 per pump) Integrated priming valves for easy solvent changes Two Integrated Push Button Manual valves for Analytical and prep Injections

Varian 445-LC Scale Up Module( SUM) - FAB Feature Advantage Benefit 2 Solvent Selection valves for solvent switching and flexibility Can prime up 4 most popular solvents thus saving time on re-priming Simplicity and Dependability Analytical and Prep Columns Positions for Automated Scale Up or Switching Seamless and simple transfer from an Analytical analysis to Prep Simplicity and Versatility Integrated Manual Valves which are dedicated for Analytical and Prep Push button valve positioning with automatic return, eliminate operator error Even Simpler to use, eliminates user error improving Confidence in results

Varian 940-LC Software Usability Software designed for Prep users Enhanced manual control One page status and chromatogram Real time fraction collection display Advanced peak sensing for better fraction collection Purity displays for absorbance detection – standard! Documentation and audit trails for traceability and regulatory needs

Varian 940-LC Configurations pump inject detect SUM FC Modular Isocratic 25ml Opt any Manual UV/Vis NA Base Grad Man inj Dual UV option Productive 1 Opt 50 Auto 5ml +man yes Productive 2 PDA Prep 100ml Opt 200 manual Dual UV/Vis

Varian 940-LC Consumables Columns designed for Prep users From 3 to 10mm media Wide range of C4 to C18 Silica and Polymer media From 2mm to 2” ID Unique solutions for dedicated prep with load and lock systems Matched Analytical to Prep columns and packing's to assure smooth scale up Solutions for hardware, software and consumables

Varian 940-LC Summary The 940-LC one instrument – many uses High performance automated Analytical HPLC High performance semi-preparative HPLC Versatility Pumping system able to operate at analytical flow rates and scalable up to prep flows Dual path-length flow cell technology for unmatched range Scale-up Module for automated methods transfer Advanced fraction collection with full software control Complete range of columns and consumables Prep enhanced Galaxie TM software and valve automation for genuine unattended operation

Conclusion for 900 Series Compact and Stylish Integrated Systems Retain flexibility and maintenance features Top Tier hardware performance Top Tier User Interface – pleasant experience Simplicity in a box – Install , Use and Maintain 1st Truly Integrated HPLC’s on the Market 26 26

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