Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells  Pu Zhang, M.D., Ph.D., Erja Kerkelä, Ph.D., Heli Skottman, Ph.D., Lev Levkov, M.D., Ph.D., Katja Kivinen, Ph.D., Riitta Lahesmaa, M.D., Ph.D., Outi Hovatta, M.D., Ph.D., Juha Kere, M.D., Ph.D.  Fertility and Sterility  Volume 87, Issue 3, Pages 677-690 (March 2007) DOI: 10.1016/j.fertnstert.2006.07.1509 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Genes detected in human GV oocytes. A–C, Correlation coefficients (r) between the replicate samples of human GV oocytes (A; r = 0.95), human embryonic stem cells (B; r = 0.93), and human fibroblasts (C; r = 0.94). D, Classification of genes expressed in human GV oocytes by biologic function. Biologic function annotation of genes was made with the use of NetAffx database (Affymetrix). E, Detection of MATER, NPM2, ZAR1, and FIGLA in human GV oocytes by RT-PCR. The order of the lanes (from left to right) is ladder, MATER, NPM2, ZAR1, and FIGLA. Beta ACTIN was used at the same time as a positive control. None of the PCR negative controls produced a visible product on the gel (data not shown). Zhang. Gene expression in human oocytes. Fertil Steril 2007. Fertility and Sterility 2007 87, 677-690DOI: (10.1016/j.fertnstert.2006.07.1509) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Twelve gene expression patterns in hGVO, hESC, and hFIB. Genes up-regulated in hGVO are shown in A, B, and C. Genes down-regulated in hGVO are shown in D, E, and F. The dashed line represents genes that are expressed in all 3 cell types; the solid line represents genes that are absent in 1 or 2 cell types but are expressed in the rest. The overlap of the solid line with the X-axis means genes were absent in that particular cell type. Inclining or declining line between 2 cell types means >2-fold increase or decrease in the expression level between the 2 cell types; a horizontal line means <2-fold difference in the expression level between the 2 cell types. The number of genes in each pattern is given besides each line. See detailed explanation in the text. Zhang. Gene expression in human oocytes. Fertil Steril 2007. Fertility and Sterility 2007 87, 677-690DOI: (10.1016/j.fertnstert.2006.07.1509) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Gene distribution on chromosomes. The white column represents the total number of human genes that are mapped to each chromosome with NCBI human genome build 35.1. The black column represents the number of genes expressed in hGVO on each chromosome. Zhang. Gene expression in human oocytes. Fertil Steril 2007. Fertility and Sterility 2007 87, 677-690DOI: (10.1016/j.fertnstert.2006.07.1509) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions