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From: Immunoproteomic Analysis of Potential Serum Biomarker Candidates in Human Glaucoma Invest. Ophthalmol. Vis. Sci.. 2012;53(13):8222-8231. doi:10.1167/iovs.12-10076 Figure Legend: Serum immunoreactivity to retinal proteins. To test serum immunoreactivity by Western blot analysis, retinal proteins were separated by SDS-PAGE, and membranes were cut into strips to probe with different serum samples. (A) Glaucomatous serum samples (1–5) exhibited a greater immunoreactivity to retinal proteins obtained from glaucomatous human donors (G) relative to retinal proteins obtained from non-glaucomatous controls (C). (B) We also tested serum immunoreactivity to retinal protein samples obtained from cell cultures. Immunoreactivity of the glaucomatous patient sera (1–5) against oxidatively stressed retinal cell culture proteins was greater than their immunoreactivity to controls. Bar graphs show fold increase in serum immunoreactivity to glaucomatous human retinal proteins (relative to nonglaucomatous retinal proteins), or fold increase in serum immunoreactivity to oxidatively stressed retinal proteins (relative to control proteins) as calculated based on band intensities. Serum dilution was 1:500, and the secondary antibody incubation used anti-human IgG conjugated with horse-radish peroxidase (1:2000). Date of download: 12/17/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.