Additional file 3. Total and percentage of mapped and unmapped RNA-seq reads generated from four successive subapical internodes of bioenergy sorghum genotype R.07020 at 60 DAP (days after planting). The RNA-seq reads were mapped to the sorghum Sbicolor-v3.1 reference sequence (Phytozome) comprising 47,205 protein-coding transcripts.    Internode samples Int1 Int2 Int3 Int4 Mean Total reads 41,142,734 35,857,428 34,642,997 41,517,811 38,290,242 Counted fragments (%) 91.9 93.8 94.4 93.9 93.5 -unique fragments (%) 88.4 90.5 91.3 90.8 90.2 -non-specifically (%) 3.5 3.4 3.1 3.3 Uncounted (%) 8.1 6.2 5.6 6.1 6.5