Blood smear.

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Presentation transcript:

Blood smear

Blood smear: A drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide. Stained with Wright’s stain.

Blood smear：Wright X400 Erythrocytes: 7-8um Biconcave disc Stain uniformly with eosin Neutrophil: proportion - 50-70%; Most numerous Multilobed nucleus Pale pink granules The method of producing a blood smear: a drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide. A smear from peripheral blood.The cells are mainly erythrocytes. Blood smear：Wright X400

Eosinophils. Blood smear：Wright X400 8-10 microns morphology 2-4% 8-10 microns morphology bilobed nucleus large, uniform eosinophil granules. Blood smear：Wright X400

Blood smear：Basophil Giemsa stain X400 Basophils. a.Proportion 0.5% b.morphology - large, irregular basophil granules obscure the nucleus The basophil granules are very large and irregularly shaped. Granules obscure the nucleus. Blood smear：Basophil Giemsa stain X400

Blood smear：Wright X400 Lymphocyte: Intensely stained nucleus Possess a slight indentation Small amount of cytoplasm Pale blue Blood smear：Wright X400

Blood smear：Wright X400 Monocyte nucleus ovoid to kidney shaped blue-gray Cytoplasm Blood smear：Wright X400

Blood smear：Wright X400 Platelets: Small speck-like objectives Aggregated into small groups From megakaryocytes in myeloid tissue. Platelets are indicated by arrows. Some leukocytes are present. Blood smear：Wright X400

Blood smear：Wright X400

Thymus Lymph node Spleen Immune system Thymus Lymph node Spleen

Capsule Interlobular septa Incomplete lobule Cortex medulla Thymus: HEX40

Capsule Interlobular septa Incomplete lobule Cortex medulla Thymus: HEX100

Thymocyte Epithelial reticular cell Thymic corpuscle Thymus: HEX400

Spheroid or kidney shaped organ Hilum Cortex Medulla Lymph node

Capsule Cortex Lymphoid nodules Paracortical zone Cortical lymph sinuses subcapsular peritrabecular Lymph node: HEX40

Small lymphocytes Germinal center young lymphocytes reticular cells macrophages Lymphoid nodule

Postcapillary venules In the paracortical zone LC within the wall thick endothelial cell LC within the wall opening for LC enter LN

Lymph node:HEX400 cortical lymph sinuses (subcapsular)

Cortical lymph sinuses: peritrabecular sinuses

Afferent lymphatic vessel in the capsule: with valves

Efferent lymphatic vessels Blood vessels Hilum of lymph node

Pacinian corpuscle Nerve fiber bundle

Medullary cords Medullary sinuses Medulla of lymph node

Spleen

Capsule DCT Smooth muscle mesothelium Spleen

Trabeculae artery vein Trabecular artery Trabecular artery Spleen

White pulps Lymphatic sheath lymphatic tissue central artery Splenic nodule Trabecular vein

White pulps Lymphatic sheath lymphatic tissue central artery Splenic nodule

Red pulps splenic cord Splenic sinusoid

Postcapillary venules in inner cortex

SS: subcapsular sinus T: trabecular

Capsule Subcapsular sinuses Trabeculae

Afferent lymph vessels GC

Older adult thymus has large amounts of adipose tissue

THE END!