Lesson 1-4 Biological Safety David LaRowe - RCC

Biosafety Emphasis Recognition of diseases transmitted by blood HIV Hepatitis Biohazard symbol

Biosafety Emphasis Biological hazard icon

Evolution of Biological Safety Guidelines Isolation Techniques (1970, CDC) Universal Precautions (1985) Bloodborne Pathogens Standard (BBP) (1991) See Table 1-14

Evolution of Biological Safety Guidelines Standard Precautions (1996) Needlestick Prevention Act (2000) Hand Hygiene (2002, 2009) See Table 1-14

Standard Precautions Comprehensive Apply to all patients Apply to all body fluids Apply to nonintact skin and mucous membranes Apply to organs and unfixed tissues See Table 1-16

Standard Precautions

Exposure Control Plan Employer responsibility Identify employees at risk Provide training Provide safety supplies Immunize workers Implement plan See Table 1-15

Exposure Control Plan Safety Agreement Form

Exposure Control Plan Plan includes use of Standard Precautions Personal protective equipment (PPE) Engineering controls Work practice controls See Table 1-17

Personal Protective Equipment Face protection Mask Safety glasses, goggles Full face shield Gloves Gown

Personal Protective Equipment

Personal Protective Equipment

Engineering Controls Splash shields Sharps container (puncture-proof) Biohazard container Safety devices Safety needles

Engineering Controls

Work Practice Controls Hand hygiene Use safety needles Immediately discard used sharps Clean up spills

Work Practice Controls

Work Practice Controls

Infection Prevention Healthcare-associated infections (HAI) Use PPE Gloves Face protection Protective coat, apron, or gown Hand hygiene Waterless antiseptics Antiseptic soap See Table 1-19

Using Exposure Control Methods Use for every task, such as routine venipuncture See Table 1-18 Observe Standard Precautions Wear appropriate PPE Use engineering controls Use work practice controls

Exposure Incidents Action plan for accidental exposure Clean wound Report to supervisor Get medical attention Employer responsibility Nonpunitive policy Immediate treatment and follow-up Confidential counseling

Microbiology Safety Cultures and specimens Decontamination See Table 1-20 Decontamination Sterilization Disinfection Antisepsis

Microbiology Safety Aseptic technique Class II safety cabinets

Laboratory Environment Air quality Humidity Temperature Air exchange Instrument performance Daily records Federal regulation This project was funded at $3,000,000 (100% of its total cost) from a grant awarded under the Trade Adjustment Assistance Community College and Career Training Grants, as implemented by the U.S. Department of Labor’s Employment and Training Administration. Rogue Community College is an equal opportunity employer/program. Auxiliary aids and services, alternate form and language services are available to individuals with disabilities and limited English proficiency free of cost upon request.   This work is licensed under a Creative Commons Attribution 4.0 International License.