BIOL 100L Microscope & Cells.

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Presentation transcript:

BIOL 100L Microscope & Cells

Microscope, Cells & Mitosis Lab Prelab Quiz Models: cells Prepared slides in order Crossed threads- just focus Letter “e”- draw at 4, 10, 40x mag 6 slides- draw at best mag include sperm and blood at 100x Wet Mount slides Pond water Yeast Cheek cell

Carrying a Microscope A B

Storing The Microscope Return the lowest power objective in place Wrap the cord around the base Return dustcover

Cleaning the Microscope Use lens paper on all glass parts of the microscope. Clean oil immersion lens with chemicals provided by your instructor

Parts of the Microscope Ocular Body tube Nosepiece Objective lenses (10, 40, 100x) Arm Course adjustment knob Fine adjustment knob Stage Stage clips Aperture Diaphragm Light Base

Microscope Parts Ocular Body tube Head Revolving nose piece Objective Arm Stage Diaphragm Lever to move stage clip Course adjustment Fine adjustment Light source Base

Using the microscope Always observe using the LOWEST POWER Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Then use FINE ADJUSTMENT KNOB . Focus, and then move to a higher power objective, if needed. Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE. Keep both eyes open to reduce eyestrain. Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

Preparing a slide

Oil Immersion Lens 40x 100x When finished: clean objective and prepared slide with lens paper

Animal Cell

Plant Cell

Chose a total of 6 prepared slides and draw at best magnification Use oil for sperm and blood

4x 10x 100x

Some slides may have more than one tissue type Adipose Hyaline cartilage

Pseudostratified ciliated columnar epithelium

Blood (RBC & WBC) use oil

sperm use oil

Cheek Cell 0.9% saline

Cheek Cell Scrape inside cheek Swirl toothpick with cells in 0.9% saline Place a small drop 0.01% methylene blue on saline saline

Cheek Cell Place coverslip Observe under microscope Draw what you see Clean up

Cheek Cell

Dispose Sharps

Clean Up

Microscope Parts Quiz

Microscope Quiz Which objective uses oil? If your ocular is 10x and your objective is 40x what is the total magnification of your image? Why do you need a cover slip and how do you avoid an air bubble? Where do you dispose slides?