Autophagy Contributes to the neuroprotection of Nampt Li Siying 8/30/2018

Glossary 1 Cerebral Ischemia 2 Autophagy 3 Nicotinamide phosphoribosyltransferase, Nampt 8/30/2018

Background Moderate Autophagy is neuroprotective1,2 Nampt is an endogenous protector against cerebral ischemia through inhibiting neuronal apoptosis and necrosis3 [1] Yue Z, Wang Q J, Komatsu M. Neuronal autophagy: going the distance to the axon[J]. Autophagy. 2008, 4(1): 94-96. [2] Shacka J J, Roth K A, Zhang J. The autophagy-lysosomal degradation pathway: role in neurodegenerative disease and therapy[J]. Front Biosci. 2008, 13: 718-736. [3] Wang P, Xu T Y, Guan Y F, et al. Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway[J]. Ann Neurol. 2011, 69(2): 360-374. 8/30/2018

Materials and Methods 1 2 3 8/30/2018 In vivo model: Middle cerebral artery occlusion (MCAO) in rats In vitro model: Oxygen-glucose deprivation (OGD) in cultured cortical neurons Nampt overexpression or knock-down by lentivirus-mediated gene transfering in vivo or in vitro immunochemistry electron microscope immunoblotting assays 8/30/2018

Results Figure1. Nampt overexpression up-regulates autophagy in the early phase of cerebral ischemia in rat MCAO model 图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. 8/30/2018

Results Figure2. Nampt regulates autophagy at the early phase of ischemic-like injury in cultured cortical neurons exposed to OGD stress LV-shRNA-Nampt: Nampt knock down 图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. 8/30/2018

Results Figure3. Autophagy contributes to the neuroprotective effect of Nampt 3-MA, 3-methyladenine: autophagy inhibitor rapamycin: autophagy inducer 图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. 8/30/2018

Conclusion 图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. 8/30/2018

Subsequent Results and Discussion 图片来源于Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. 8/30/2018

References [1] Wang P, Guan Y F, Du H, et al. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia[J]. Autophagy. 2012, 8(1): 77-87. [2] Yue Z, Wang Q J, Komatsu M. Neuronal autophagy: going the distance to the axon[J]. Autophagy. 2008, 4(1): 94-96. [3] Shacka J J, Roth K A, Zhang J. The autophagy-lysosomal degradation pathway: role in neurodegenerative disease and therapy[J]. Front Biosci. 2008, 13: 718-736. [4] Wang P, Xu T Y, Guan Y F, et al. Nicotinamide phosphoribosyltransferase protects against ischemic stroke through SIRT1-dependent adenosine monophosphate-activated kinase pathway[J]. Ann Neurol. 2011, 69(2): 360-374. 8/30/2018