Figure 3 3-MA does not inhibit the formation of autophagosomes induced by ZnPPIX HeLa cells were pre-incubated with either 0.1% DMSO (control) or 10 mM.

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Figure 3 3-MA does not inhibit the formation of autophagosomes induced by ZnPPIX HeLa cells were pre-incubated with either 0.1% DMSO (control) or 10 mM 3-MA for 2 h before treatment with either ZnPPIX (10 μM), Z18 (10 μM), or rapamycin (500 nM). (A) After combination treatment for 12 h, autophagy activity was detected by A1R-si laser scanning confocal microscopy. There was no difference in ZnPPIX-treated cells with or without 3-MA. (B,C) The average number of GFP-LC3 puncta per cell (B) and the percentage of cells with an obvious accumulation of GFP-LC3 puncta (C) after ZnPPIX treatment were quantified using ImageJ. There was no difference in ZnPPIX-treated cells with or without 3-MA. Data are expressed as mean ± SD from at least three independent experiments. *P< 0.05. (D) Western blot results showed that, after combination treatment for 12 h, 10 mM 3-MA has no effect on the conversion of LC3-I to LC3-II in ZnPPIX-treated HeLa cells. From: The heme oxygenase-1 inhibitor ZnPPIX induces non-canonical, Beclin 1-independent, autophagy through p38 MAPK pathway Acta Biochim Biophys Sin (Shanghai). 2012;44(10):815-822. doi:10.1093/abbs/gms064 Acta Biochim Biophys Sin (Shanghai) | © The Author 2012. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. 1