Array technology permits simultaneous quantification of dozens to millions of analytes. In the array-based rtPCR panel depicted here, RNA is extracted.

Slides:



Advertisements
Similar presentations
Date of download: 5/28/2016 Copyright © The American College of Cardiology. All rights reserved. From: Expression, activity and functional significance.
Advertisements

Two examples of incised wounds
Complex atypical hyperplasia of the endometrium
Abdominal aortic aneurysm (AAA or "triple A")
Large cell undifferentiated carcinoma of lung
Anatomy of the breast (diagrammatic sagittal section)
Calcific aortic stenosis
Desmoplastic small round cell tumor
Individual private insurance
Tenosynovial giant cell tumor Left low power view shows a lobulated tumor mass with lobules divided by thick collagen fibers (left, low-power view). High.
Normal gastric mucosa. (A) The epithelial component of the gastric mucosa consists of surface foveolar epithelium that is connected to the deep secretory.
Nine patterns of inflammatory skin disease. (See also Table 8–1.)
Diagnostic algorithm for normocytic anemia.
Angiosarcoma. (A) The dermis has been largely replaced by highly atypical cells in this patient with angiosarcoma. (B) Malignant cells form bizarre vascular.
Traditional gene expression microarray schematic
Pathophysiology of alcoholic liver disease
Papillary serous carcinoma of the ovary
Abnormal intracellular accumulations: (A) Fatty change in liver (alcoholic steatosis): Tissue processed for H&E staining (lipid extracted). (Inset): Frozen.
Various developmental diverticulitis of the small bowel
Primary biliary cirrhosis
Chronic hepatitis C infection
Ulcerative colitis. Note the continuous segment of mucosal erythema extending from the distal aspect of the specimen (right of image). This pattern of.
Vasculitis versus vessel caliber
Midsagittal section through the brain of a patient with a brain stem tumor. Histologic findings showed the tumor to be an ependymoma. Source: Discussion.
Patterns of ductal carcinoma in situ (DCIS)
Human papillomavirus (HPV) type 16 DNA demonstrated in a cervical smear by in situ hybridization. The dark dots represent detection of HPV DNA sequences.
Rodin, “The Kiss” (545 x 800). Accessed at
Overview of eicosanoid synthesis: Some anti-inflammatory eicosanoids use parallel pathways substituting dihomo-gamma-linolenic acid (DGLA), a derivative.
Illustration of ways regression lines can differ
The biopsychosocial (BPS) model of disease provides an important context for the patient’s experience (a) In a purely biomedical model, pathology explains.
Pancreatic lobule. Grossly and microscopically normal pancreatic tissue has a lobular architecture. The majority of the lobule is composed of acinar tissue.
Outline of the process of TMA assembly
CMMl. PB smear (A, Wright stain, ×600)
Metastatic colon cancer
(A) Region of esophagus demonstrating Barrett esophagus: The normal squamous epithelium (left margin of figure) is replaced by specialized columnar epithelium.
Columnar cell lesions and atypical ductal hyperplasia
The processing of microRNA that is involved in gene regulation by RNA interference. (Reprinted with permission from Brooker RJ: Genetics: Analysis & Principles,
Decision tree for ulcerative colitis
Semilog graph of the rate of drug elimination in a one-compartment model. Source: One-Compartment Open Model: Intravenous Bolus Administration, Applied.
A Pavlik harness is used to treat infants with developmental dysplasia of the hip. Source: Orthoses for Paraplegia or Hip Impairments, Prosthetics & Orthotics.
Marrow production levels following iron dextran injection
Electrical burn on the foot of an individual who came into contact with a source of high voltage. There were corresponding burns on the sock and shoe,
Hierarchy of clinical evidence
Cut-away view of a modern MRI machine. Please see text for description. Source: Overview of Diagnostic Modalities and Contrast Agents, Introduction to.
A. Use of athletic tape to make an extension block for the wrist. B
Sickle cell anemia. This peripheral blood smear exhibits sickled cells (arrowheads). Sickled cells pass through the vasculature with more difficulty, becoming.
An emerging molecular “parts list” for schizophrenia
Ischemic colitis. This example of ischemic colitis shows mucosal hemorrhage, necrosis of the superficial portions of the crypts, and lamina propria hyalinosis.
Gastrointestinal stromal tumor
Anatomy of the colon. (A) Representation of the anterior portion of the colon. The right and left colic flexures are commonly referred to as the hepatic.
The straight leg knee immobilizer brace
World Health Organization (WHO) three-step ladder oral analgesic program for managing cancer pain. Source: Chapter 48. Pain Management and Symptom Control,
Molecular characterization of the sinus node at the late fetal stage in the mouse, as assessed by in situ hybridization of mRNA for connexin 40 (left)
[From: Galli : et al: Emergency Orthopedics: The Spine
Iron deficiency anemia
Photomicrograph of bone biopsy with metastatic prostate adenocarcinoma
Follicular cysts. (A) Clinical presentation of follicular cysts
Number of uninsured persons in the United States, 1980 to 2014 (U. S
 The human genome contains approximately genes.  At any given moment, each of our cells has some combination of these genes turned on & others.
Clinical and pathologic effects of hepatocellular failure, which commonly results from conditions associated with acute or chronic necrosis of liver cells.
Acute viral hepatitis. Acute viral hepatitis is most commonly characterized by lymphocyte predominant inflammatory infiltrates and foci of hepatocyte injury.
Example of a DNA Array (note green, yellow red colors; also note that only part of the total array is depicted)
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
Expression of Two Breast-Specific Molecules in the Lung
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
An Array-Based Analysis of MicroRNA Expression Comparing Matched Frozen and Formalin-Fixed Paraffin-Embedded Human Tissue Samples  Xiao Zhang, Jiamin.
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
Copyright © 2004 The McGraw-Hill Companies, Inc. All rights reserved.
Presentation transcript:

Array technology permits simultaneous quantification of dozens to millions of analytes. In the array-based rtPCR panel depicted here, RNA is extracted from paraffin-embedded tissue and then spiked with a control sequence before being reverse transcribed to cDNA. The cDNA is divided among wells for real-time rtPCR measuring multiple separate RNAs (e.g., to classify RNA expression profile, or to detect multiple RNA viruses). Source: Clinical Practice: Molecular Pathology, Pathology: A Modern Case Study Citation: Reisner HM. Pathology: A Modern Case Study; 2015 Available at: http://accessmedicine.mhmedical.com/DownloadImage.aspx?image=/data/Books/1569/rei_ch5_f007.png&sec=95968897&BookID=1569&ChapterSecID=95968854&imagename= Accessed: December 19, 2017 Copyright © 2017 McGraw-Hill Education. All rights reserved

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.