Serological diagnosis of Toxoplasma gondii infection

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Presentation transcript:

Serological diagnosis of Toxoplasma gondii infection O. Villard, B. Cimon, C. L’Ollivier, H. Fricker-Hidalgo, N. Godineau, S. Houze, L. Paris, H. Pelloux, I. Villena, E. Candolfi Diagnostic Microbiology and Infectious Disease Volume 84, Issue 1, Pages 22-33 (January 2016) DOI: 10.1016/j.diagmicrobio.2015.09.009 Copyright © 2016 The Authors Terms and Conditions

Fig. 1 Advice algorithm for toxoplasma serology with negative IgG and IgM. Adapted from Villard et al., 2011. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 2 Serological monitoring for toxoplasma serology with positive IgG and negative IgM. Adapted from Villard et al., 2011. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 3 Serological monitoring for toxoplasma serology with negative IgG and positive IgM. Adapted from Villard et al., 2011. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 4 Serological monitoring for toxoplasma serology with positive IgG and IgM. Adapted from Villard et al., 2011. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 5 Advice algorithm for toxoplasma serology with equivocal IgG and negative IgM. Adapted from Villard et al., 2011. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 6 IgG immunoblot. 1. Positive control with 5 bands corresponding to 30, 31, 33, 40, and 45kDa. 2. False-positive serum with an equivocal titer of IgG. 3. Positive patient with the 5 bands. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 7 Advice algorithm for toxoplasma serology in children born from mother with confirmed or highly suspected contamination during pregnancy. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 8 Comparison of immunoblot IgG and IgM with paired mother (M) and infant (i) at birth. 1. Absence of IgM and same profile of IgG in favor of passive transmission of maternal IgG. 2. Same profile for IgG and different profile IgM (arrows) confirming congenital toxoplasmosis. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 9 Advice algorithm for toxoplasma serology in patients with a suspected toxoplasma chorioretinitis. Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions

Fig. 10 Advice algorithm for toxoplasma serology in immunosuppressed patients. Patients with AIDS (A), SOT (B), and HSCT (C). Diagnostic Microbiology and Infectious Disease 2016 84, 22-33DOI: (10.1016/j.diagmicrobio.2015.09.009) Copyright © 2016 The Authors Terms and Conditions