Mitochondrial Ferritin Protects Hydrogen Peroxide-Induced Neuronal Cell Damage Guofen Gao 1 ;Nan Zhang 1 ;Yue-Qi Wang 1 ;Qiong Wu 1 ;Peng Yu 1 ;Zhen-Hua.

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Mitochondrial Ferritin Protects Hydrogen Peroxide-Induced Neuronal Cell Damage Guofen Gao 1 ;Nan Zhang 1 ;Yue-Qi Wang 1 ;Qiong Wu 1 ;Peng Yu 1 ;Zhen-Hua Shi 1 ;Xiang-Lin Duan 1 ;Bao-Lu Zhao 1 ;Wen-Shuang Wu 2 ;Chang Yan-Zhong 1 ; 1 Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China ; 2 The 3rd Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, China ; Figure 2. FtMt protected cells from apoptosis by maintaining Bcl-2Bax ratio and inhibiting activation of caspase 3 A The protein expression levels of Bcl-2 and Bax in H 2 O 2 treated 100 #cod#x003BC;M, 24 h or untreated cells were determined by western-blot right panel, and ratios of Bcl-2Bax were calculated and presented as mean #cod#x000B1; SD left panel; n=6 #cod#x0002A;, P #cod#x0003C; 0.05 and #cod#x0002A;#cod#x0002A;, P #cod#x0003C; 0.01 vs. the untreated cells of same genotype; ##, p #cod#x0003C; 0.01 vs. the H 2 O 2 -treated vector controls. B Expression levels of caspase 3 and cleaved-caspase 3 were shown right panel. Ratios of cleaved-caspase 3caspase 3 were calculated and presented as mean #cod#x000B1; SD left panel; n=6 #cod#x0002A;#cod#x0002A;, P #cod#x0003C; 0.01 vs. the untreated cells of same genotype; ##, p #cod#x0003C;0 .01 vs. the H 2 O 2 -treated vector controls. Aging and Disease,null,8(4),458-470. Doi:10.14336/AD.2016.1108