Quick, simple method creation using Venus

Venus – Programming Levels Two levels of programming within Venus Graphically based tools to quickly create simple methods Deck Editor Action Editor SMART steps Graphically based tools to quickly create simple methods Deck Editor Action Editor SMART steps Full access to range of features to build methods that include Custom user interfaces Worklists for sample information Control of Hamilton or 3rd party devices i.e. plate readers Sample tracking using barcodes Full access to range of features to build methods that include Custom user interfaces Worklists for sample information Control of Hamilton or 3rd party devices i.e. plate readers Sample tracking using barcodes

Venus – Graphical Programming Deck Editor Tool for creating a deck layout A deck layout is required for each method so venus knows where to pipette or move plates Either modify an existing layout or create a new one Venus comes with set of labware installed including carriers and plates Tool for creating a deck layout A deck layout is required for each method so venus knows where to pipette or move plates Either modify an existing layout or create a new one Venus comes with set of labware installed including carriers and plates More Details

Venus – Graphical Programming Creating a Deck Layout Three ways to find labware Labware Categories – drill down layers Search – type in labware name Browse – drill down through Hamilton Labware folder Three ways to find labware Labware Categories – drill down layers Search – type in labware name Browse – drill down through Hamilton Labware folder Note – Labware created during your installation can be found in the Hamilton labware folder C:\Program Files\Hamilton\Labware\MyName

Venus – Graphical Programming Creating a Deck Layout To create a deck Click on labware and drag labware onto deck Yellow triangles at corners indicate labware at a snap to fit position Click on labware and drag labware onto deck Yellow triangles at corners indicate labware at a snap to fit position

Venus – Graphical Programming Action Editor Tool for method planning Drag action from Toolbox to between Start and End blocks to create assay workflow Insert step into an action to perform pipetting or plate movement Action Editor Tool for method planning Drag action from Toolbox to between Start and End blocks to create assay workflow Insert step into an action to perform pipetting or plate movement More Details

Venus – Graphical Programming Action Editor To insert action Drag action from Toolbox into action area between Start and End blocks Drag action from Toolbox into action area between Start and End blocks

Venus – Graphical Programming Action Editor To insert step Select step from Insert Step dropdown box Can only insert step into new action Select step from Insert Step dropdown box Can only insert step into new action

Venus – Graphical Programming SMART steps Wizards to guide you through setting up a pipetting process Simple (1-1) – transfer from one source well to one destination well Replica (1-n) - transfer from one source well to multiple destination wells Pooling (n-1) - transfer from multiple source wells to one destination well Aliquot – multidispensing from single aspirate Simple (1-1) – transfer from one source well to one destination well Replica (1-n) - transfer from one source well to multiple destination wells Pooling (n-1) - transfer from multiple source wells to one destination well Aliquot – multidispensing from single aspirate

Venus – Graphical Programming SMART steps Information required Where to aspirate from Where to dispense to Volume Tip type Liquid class Where to aspirate from Where to dispense to Volume Tip type Liquid class More Details

Venus – Graphical Programming SMART steps Select Where to aspirate from Where to dispense to Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish. Where to aspirate from Where to dispense to

Venus – Graphical Programming SMART steps Select Where to aspirate from Where to dispense to Volume Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

Venus – Graphical Programming SMART steps Select Where to aspirate from Where to dispense to Volume Tip type Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

Venus – Graphical Programming SMART steps Select Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

Venus – Graphical Programming SMART steps Select Where to aspirate from Where to dispense to Volume Tip type Dispense mode and liquid class If source and destination don’t contain the same number of wells, use schematics to decide if using all the aspirate or dispense wells will cause the SMART step to finish.

Venus – Graphical Programming Drag and drop programming of SMART steps Concept enables user to select which wells to aspirate from or dispense to during creation of SMART step Simply click on wells to be used and drag into appropriate box within SMART step Concept enables user to select which wells to aspirate from or dispense to during creation of SMART step Simply click on wells to be used and drag into appropriate box within SMART step More Details

Venus – Graphical Programming Drag and drop programming Need to have SMART step open with Deck Editor on sequences tab Click on wells to be aspirated/dispensed Need to have SMART step open with Deck Editor on sequences tab Click on wells to be aspirated/dispensed Selected wells are Brown

Venus – Graphical Programming Drag and drop programming Hover mouse over selected wells Hold down control and drag into appropriate box in SMART step Hover mouse over selected wells Hold down control and drag into appropriate box in SMART step Drag into box Hover mouse

Venus – Graphical Programming Drag and drop programming Depending on system configuration settings either a default name appears in box or user is asked to enter a name for wells used Wells used now change colour Depending on system configuration settings either a default name appears in box or user is asked to enter a name for wells used Wells used now change colour Default name created Wells used in aspirate step are now dark blue

Venus – Graphical Programming Quick method creation – putting it all together Create deck layout With this approach Select wells required for each SMART step created. Two videos with information sheets available demonstrating this approach. Distribute buffer using Aliquot SMART step Plate to plate transfer using Simple (1-1) SMART step With this approach Select wells required for each SMART step created. Two videos with information sheets available demonstrating this approach. Distribute buffer using Aliquot SMART step Plate to plate transfer using Simple (1-1) SMART step Insert action block Plan method in Action Editor Insert SMART step into Action Block Fill out SMART step wizard Test in simulation mode