Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós.

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Presentation transcript:

Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós Electronic Engineering Department Universitat Politècnica de Catalunya

Contents 1.- Introduction 2.- Motivation 3.- Multitone Burst Signal 4.- Results 5.- Conclusions

Introduction Linear Time Invariant system: Impedance Transfer Function Laplace/Fourier Transform 1

Introduction Biological systems: Bioimpedance 2 Equivalent Circuit Cell membrane Extracellular matrix LF HF f R 8 |Z| Ro fc 2

Introduction Ex-vivo tissue characterization: 3

Introduction In-vitro static cell culture growth characterization: 4

Motivation Challenge (I): In-vitro myocites cell culture growing characterization for tissue engineering applications Link video 5

Motivation Challenge (II): In-vivo myocites patch monitoring and characterization: 6

Multitone Burst Signals Time domain: 7

Multitone Burst Signals Frequency domain: 8

Multitone Burst Signals Crest Factor: Newman: Schroeder: Genetic Algorithm: 9

Multitone Burst Signals Frequency Plan: Avoid intermodulation products: shift original tones for BW guard. 10

Multitone Burst Signals Multitone bursts with Bilateral Quasi Logarithmic Frequency Distribution: 11

Results Measurement Hardware Setup: Custom FPGA based arbitrary signal generator. DAC 12bits, 16Msamples, 40Ms/s, 1st order hold. Ethernet capable controller. PXI solution PXI-5422: AWG, 200Ms/s, 32MB,16 bits. PXIe-5122: AD 2channels, 100Ms/s, 64MB/channel, 14bits. PXI-2530: multiplexation matrix, 128 channels. 12

Results Yeast cell suspension settlement characterization: Estimation of the Growth Cell Curve 13

Conclusions Non invasive and non destructive technique for passive electrical properties charaterization of biological materials. Valid measurement method for cell growth estimator and suitable for dynamic biological materials and systems characterization. Wide application areas: electroporation, tomography, cell counting in microfludic, biosensors, tissue engineering,.. The final application field is the cardiac tissue characterization in regenerative medicine applications. 14

Fast Electrical Impedance Spectroscopy measurement techniques for dynamic bioimpedance characterization. Ph.D. Student: B.Sánchez Thesis Advisor: R.Bragós Electronic Engineering Department Universitat Politècnica de Catalunya