A b Goat anti-mouse IgG-Alexa 594 on sections of freshly isolated mouse metatarsal joint Biotinylated horse anti-mouse IgG on sections of human articular.

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a b Goat anti-mouse IgG-Alexa 594 on sections of freshly isolated mouse metatarsal joint Biotinylated horse anti-mouse IgG on sections of human articular cartilage c Biotinylated goat anti-rabbit IgG on sections of cultured mouse metatarsal joint d Goat anti-mouse IgG-Alexa 594 on sections of freshly isolated mouse knee joint SB AC DAPI Biotinylated horse anti-mouse IgG with Streptavidin-DyLight 594 on cultured mouse metatarsal joint e Additional Figure S1. Images of no primary antibody IHC controls. DAPI images are provided in immunofluorescence assays for orientation. (a) Control for PEDF IHC on sections of human OA articular cartilage, using biotinylated horse anti-mouse IgG. Arrows indicate articular surface. Scale bar = 200μm. (b) Control for PEDF IHC on sections of freshly isolated mouse metatarsal joint, using goat anti-mouse IgG, Alexa Fluor 594 conjugate. (c) Control for MMP-1 and MMP-3 IHC on sections of cultured mouse metatarsal joint, using biotinylated goat anti-rabbit IgG. (d) Control for MMP-13 IHC on sections of cultured mouse metatarsal joint, using biotinylated horse anti-mouse IgG, streptavidin DyLight 594 conjugate. (e) Control for PEDF IHC on sections of freshly isolated mouse knee joint, using goat anti-mouse IgG, Alexa Fluor 594 conjugate. Scale bars for b-d = 100μm. AC: articular cartilage. SB: subchondral bone.