6 Basic Steps to Completing Single Cross Punnett Squares Problem : A harmless chemical known as PTC can be added to strips of paper to test for the presence of dominant & recessive alleles. Being able to taste the chemical (T) is dominant to being a non-taster (t). If two heterozygous tasters are crossed, what are the possible genotypes and phenotypes of their children? Step #1 – Create a key showing traits as dominant/recessive. T = PTC taster t = non-taster
6 Basic Steps to Completing Single Cross Punnett Squares Step # 2 – Make a list of possible allele combinations & the phenotypes that each would produce. TT= PTC-taster Tt = PTC-taster tt = non-taster Step # 3 – Identify the parents that are being used in the cross. Tt x Tt
6 Basic Steps to Completing Single Cross Punnett Squares Step # 4 – Draw a Punnett square to determine possible offspring. T t T TT Tt t Tt tt Step # 5 – Identify the Genotypic ratio. 1 : 2 : 1 TT Tt tt Step # 6 – Identify the Phenotypic ratio. 3 : 1 PTC-tasters non-taster