Immunohistochemical expression of connexin 43 and occludin in the rat testis after epididymal and vasal ligation  Barış Altay, M.D., Burak Turna, M.D.,

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Immunohistochemical expression of connexin 43 and occludin in the rat testis after epididymal and vasal ligation  Barış Altay, M.D., Burak Turna, M.D., Gülperi Öktem, M.D., Hüseyin Aktuğ, M.D., Bülent Semerci, M.D., Ayhan Bilir, Ph.D.  Fertility and Sterility  Volume 90, Issue 1, Pages 141-147 (July 2008) DOI: 10.1016/j.fertnstert.2007.05.065 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Electron microscopic view of ipsilateral rat testicular tissue after vasal ligation (original magnification, ×3000). Cytoplasmic destruction (yellow arrow) and vacuolization (red arrow) are indicated. Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Electron microscopic view of contralateral rat testicular tissue after vasal ligation (original magnification, ×3000). Arrows indicate decreased degenerative findings in the cytoplasm (yellow arrow) and cell membrane (red arrow). Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Semiquantitative analysis of the occludin immunostaining in all groups (sham = sham-operated group; CLVL = contralateral vasal ligation group; ILVL = ipsilateral vasal ligation group; CLEL = contralateral epididymal ligation group; ILEL = ipsilateral epididymal ligation group). Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Semiquantitative analysis of the Cx43 immunostaining in all groups (sham = sham-operated group; CLVL = contralateral vasal ligation group; ILVL = ipsilateral vasal ligation group; CLEL = contralateral epididymal ligation group; ILEL = ipsilateral epididymal ligation group). Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Immunolocalization of occludin in the ipsilateral testicular tissue after vasal ligation (original magnification, ×100). Arrows show positive immunoreactivity for occludin within the germ cells. Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 6 Immunolocalization of connexin 43 in the ipsilateral testicular tissue after epididymal ligation (original magnification, ×100). Arrows show positive immunoreactivity for connexin 43 within the germ cells. Fertility and Sterility 2008 90, 141-147DOI: (10.1016/j.fertnstert.2007.05.065) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions