Nano Cost Nano Patterned Template for Surface Enhanced Raman Scattering H.-C. Hou, J. Kim, K. Kanakamedla, S Basu, T. Daniels-Race,* and M. Feldman H Division of Electrical and Computer Engineering *LSU Center for Computation & Technology Louisiana State University Baton Rouge, LA 70803 Work supported in part by the NIH

Raman Scattering

Raman Spectrometer

Surface Enhancement Raman Spectrometer (SERS) (Rhodamine 6G in DI Water)

Aluminum Foil Substrates Sample Size: 1 cm x 1 cm Sample Cost: 0.005 cents Front Side: Smooth Scatters light in 1 direction Back Side: Dull Scatters light in 2 directions Application: Conventional

Rhodamine 6G Spectra from Aluminum Foil Front Front Front Back Back Back Not Etched Etched Etched and Stirred

99.99% Pure Aluminum Pellet Not Etched Etched Raman Signal

Shop Grade Aluminum Sample Raman Signal Raman Signal Non Etched Etched

Aluminum Substrate All Aluminum Works the Same More or less Etching helps Foil Pure aluminum Shop grade aluminum

20 nm of Gold Sputtered on Aluminum Foil 20,000 10,000 Raman Signal

Sketch of Sputtering Chamber

Position in Sputtering Chamber

Position in Sputtering Chamber

Signal from Low to High Concentrations Au on Aluminum Foil Intensity (A. U. ) Concentration - μM

Gold Further Enhances Aluminum Signal A Little Gold Goes a Long Way (ancient saying) Especially at edge of sputtering chamber

Clinical Applications Conventional Remote Probe

Conventional vs Probe

Remote Probe

Couple GRIN Lens to Spectrometer

Fabrication of Probe (or GRIN Lens)

Raman Spectra Conventional Probe Intensity (A.U.) Intensity (A.U.) Raman Shift (cm-1) Raman Shift (cm-1) Conventional Probe 21

Raman Signal Through Epoxy and Gold

Shape of Exposed Gold May Change u m i E x y

Probe in Gelatin Block- 1.1 meter from Spectrometer

Spectrum – 1mM Rhodamine in Gelatin

Signal in Cell from Low to High Concentrations Au on Epoxy Probe Intensity (A. U. ) Concentration - μM

In Situ Application of Probe

Biologic Results After Vardaki et al. Our Chicken Results Chicken Bone After Vardaki et al. University of Patras, 2013 Our Chicken Results

Thank you!

Raman Signal vs. Angle of Incidence Not Etched Pellet

Au on Glass

AFM and SEM Au on Glass

AFM images of Aluminum (F) Non Etched Etched

AFM images of Aluminum (B) Non Etched Etched

AFM Au on etched Al (F)

AFM and SEM Au on etched and stirred Al (F) Scale Bar 300nm 1= 30nm 1.3 um x 1.3 um Scale 25nm 37

AFM Au on Epoxy (Stirred and Etched Al Removed)